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putR
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putR

transcriptional activator of the proline utilization operon [gene|1F548515402950572E7212901729B2480432D1B9|putB]-[gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]-[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]
locus
BSU_03230
pI
5.37
mw
47.67 kDa
protein length
411 aa Sequence Blast
gene length
1236 bp Sequence Blast
function
regulation of proline utilization
product
transcriptional activator ([SW|PucR family])
essential
no
synonyms
ycgP,prcR

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.2|Utilization of proline]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • Gene

    Coordinates
    348,724 → 349,959

    Phenotypes of a mutant

  • no growth with proline as single source of carbon or nitrogen [Pubmed|21840319]
  • The protein

    Catalyzed reaction/ biological activity

  • transcription activation of the proline utilization operon ''[gene|1F548515402950572E7212901729B2480432D1B9|putB]-[gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]-[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]'' in the presence of proline [Pubmed|21840319,21964733]
  • Protein family

  • [SW|PucR family]
  • [SW|CdaR family] (according to UniProt)
  • [SW|Cofactors]

  • proline acts as co-activator [Pubmed|21840319]
  • Effectors of protein activity

  • proline activates PutR [Pubmed|21840319]
  • Expression and Regulation

    Operons

    genes
    [gene|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR]
    description
    [Pubmed|21840319]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21964733], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulation

  • constitutively expressed [Pubmed|21840319,21964733]
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    Biological materials

    Mutant

  • BKE03230 (Δ[gene|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCTTTTTCTCCTATAC, downstream forward: _UP4_TAAAAAAGAAACCAGTACTT
  • BKK03230 (Δ[gene|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCTTTTTCTCCTATAC, downstream forward: _UP4_TAAAAAAGAAACCAGTACTT
  • References

  • 21840319,21964733,22139509