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purT
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purT

phosphoribosylglycinamide formyltransferase 2
locus
BSU_02230
pI
4.72
mw
41.93 kDa
protein length
384 aa Sequence Blast
gene length
1155 bp Sequence Blast
function
purine biosynthesis
product
phosphoribosylglycinamide formyltransferase 2
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]

    • Gene

    Coordinates
    243,892 → 245,046

    The protein

    Catalyzed reaction/ biological activity

  • ATP + formate + N1-(5-phospho-D-ribosyl)glycinamide --> ADP + H+ + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + phosphate (according to UniProt)

    • Protein family

  • PurK/PurT family (with [protein|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|PurK], according to UniProt)

    • Paralogous protein(s)

  • [protein|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|PurK]

    • [SW|Domains]

  • [SW|ATP-grasp domain] (aa 111-300) (according to UniProt)

    • Structure

  • [PDB|1KJ9] (with Mg-ATP from ''Escherichia coli'', 55% identity, 69% similarity) [Pubmed|11953435]

    • Expression and Regulation

    Operons

    genes
    [gene|C00E34EF63B5EAE33CAD5E469893C0A5112B9F35|purT]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7496533], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • BKE02230 (Δ[gene|C00E34EF63B5EAE33CAD5E469893C0A5112B9F35|purT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT, downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG
  • BKK02230 (Δ[gene|C00E34EF63B5EAE33CAD5E469893C0A5112B9F35|purT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT, downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG

    • References

  • 7496533