aag
168
hypoxanthine-DNA glycosylase, general stress protein, required for protection against paraquat stress
locus
BSU_38620
Molecular weight
21.98 kDa
pI
6.12
function
DNA repair, survival of stress conditions
product
hypoxanthine-DNA glycosylase
essential
no
synonyms
aag, yxlJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2094 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,964,278 3,964,868
Phenotypes of a mutant
increased sensitivity of sporulating cells to the genotoxic effects of MMS [Pubmed|27698084]
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The protein
Catalyzed reaction/ biological activity
binds to 8-oxoG, abasic, and RNA base DNA lesions [pubmed|37250769]
removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates [Pubmed|14729667]
protects sporulating cells from cytotoxic and genotoxic effects of MMS [Pubmed|27698084]
Protein family
DNA glycosylase MPG family (single member, according to UniProt)
Structure
[PDB|3QI5] (the human enzyme in complex with DNA, 33% identity) [pubmed|21349833]
[AF|P94378]
[wiki|Localization]
forespore [Pubmed|27698084]
Expression and Regulation
Operons
genes
[gene|305DCA94A69BCF64E377F142722A035871A3B824|aag]-[gene|9A30DA670D03D63C59DEB54EA7BD6DCEDDBD561A|yxzF]
description
[Pubmed|8990303]
regulation
expressed late during [wiki|sporulation] in the forespore [wiki|SigG] [Pubmed|27698084]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|11544224], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|27698084], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
MGNA-B756 (yxlJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1755 NBRP B. subtilis, Japan]
BKE38620 ([gene|305DCA94A69BCF64E377F142722A035871A3B824|aag]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAATCGACCTCTCCTTT, downstream forward: _UP4_TAGCATGTTTAAGGAAGAGG
BKK38620 ([gene|305DCA94A69BCF64E377F142722A035871A3B824|aag]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAATCGACCTCTCCTTT, downstream forward: _UP4_TAGCATGTTTAAGGAAGAGG
References
Reviews
Original Publications
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Time of last update: 2024-04-18 18:43:53
Author of last update: Jstuelk