yvcJ
168
GTPase, nucleotide-binding protein, similar to RNase adaptor protein
locus
BSU_34770
Molecular weight
33.70 kDa
pI
6.7
function
required for the expression of late competence genes
product
GTPase
essential
no
synonyms
yvcJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1660 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,571,501 → 3,572,388
Phenotypes of a mutant
reduced [wiki|genetic competence] [Pubmed|19074378]
defective in swarming motility (reduced rate of colony expansion) [pubmed|35638827]
Advertisement
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes nucleotides, required for the expression of late competence genes (''[gene|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK], [gene|4D76F07D6124D56C7CEBBDAB9E84B2718BF1E7FC|comS]'') [Pubmed|19074378]
Protein family
RapZ-like family (single member, according to UniProt)
Structure
[PDB|5O5Q] (RapZ from E. coli, 41% identity) [pubmed|28977623]
[AF|O06973]
[wiki|Localization]
can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium [Pubmed|21709426]
Additional information
[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=947727&log$=genesensor5&logdbfrom=pubmed Information on the ''E. coli'' homolog]
Expression and Regulation
Operons
genes
[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
description
[Pubmed|9237995]
regulation
constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]
expression
in Enterococcus faecalis: induced upon cell wall stress (vancomycin) ([protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]) [pubmed|38512984]
regulatory mechanism
[protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]: transcription repression, described for Enterococcus faecalis [pubmed|38512984], in [regulon|protein:1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16272399], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB]' [PubMed|20525796]
Biological materials
Mutant
MGNA-B641 (yvcJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1640 NBRP B. subtilis, Japan]
1A885 ( ''yvcJ''::''cat''), [Pubmed|19074378], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A885&Search=1A885 BGSC]
BKE34770 (Δ[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCTCCCCCCTGACT, downstream forward: _UP4_ATTGAAAAGAGAAGCCGGAA
BKK34770 (Δ[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34770 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTCTCCCCCCTGACT, downstream forward: _UP4_ATTGAAAAGAGAAGCCGGAA
Expression vectors
pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Stülke] lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki| Görke] lab
labs
[wiki|Anne Galinier], University of Marseille, France
References
Reviews
Original Publications
Publications on the ''E. coli'' homolog
Page visits: 2955
Time of last update: 2024-04-16 08:10:32
Author of last update: Jstuelk