mnmL
168
radical SAM enzyme, synthesis of mnm5s2U at the Wobble base position (U34) in tRNAs
locus
BSU_30480
Molecular weight
36.61 kDa
pI
5.61
function
tRNA modification
product
unknown
essential
no
synonyms
ytqA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1242 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,119,999 3,120,967
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The protein
Catalyzed reaction/ biological activity
5-carboxymethylaminomethyl-2- thiouridine --> 5-aminomethyl-(2-thio)uridine [pubmed|38551342]
synthesis of mnm5s2U at the Wobble base position (U34) in tRNAs [pubmed|38551342]
Protein family
[wiki|Radical SAM superfamily] [pubmed|38551342]
[wiki|Cofactors]
4 Fe- 4S cluster [pubmed|29292548]
Structure
[AF|O35008]
Expression and Regulation
Operons
genes
[gene|AB9762F1D388E27039FEFE946D8BE527CEF13F09|mnmL]-[gene|B7B3DF7FFA4144CBB12A72996C73CFBD7B6DD2D8|mnmM]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A129 (ytqA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/129 NBRP B. subtilis, Japan]
BKE30480 ([gene|AB9762F1D388E27039FEFE946D8BE527CEF13F09|mnmL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATTCATGTGCCTCCTT, downstream forward: _UP4_CGGCTTGAGGAGGAATCAGC
BKK30480 ([gene|AB9762F1D388E27039FEFE946D8BE527CEF13F09|mnmL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATTCATGTGCCTCCTT, downstream forward: _UP4_CGGCTTGAGGAGGAATCAGC
References
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Time of last update: 2024-04-18 18:11:35
Author of last update: Jstuelk