cmoO
168
monooxygenase, required for the conversion of S-methyl-cysteine to cysteine
locus
BSU_29330
Molecular weight
36.79 kDa
pI
5.84
function
utilization of S-methyl-cysteine
product
monooxygenase
essential
no
synonyms
cmoO, ytmO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2141 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,003,345 → 3,004,349
Phenotypes of a mutant
no growth with S-methyl cysteine [Pubmed|23944997]
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The protein
Protein family
Flavin-utilizing monoxygenases
[wiki|Cofactors]
FMN [pubmed|35584544]
Structure
[PDB|4US5] (from Streptomyces bottropensis, 31% identity) [pubmed|24554499]
[AF|O34846]
Paralogous protein(s)
[protein|A1DCFF8D225C87197104742E72A0D4F238BC30EB|ywcH], [protein|A36E7D50E2EBD0F0109491FF2CAD2CA84372B2CF|yvbT]
[protein|1432F56AD3D2B2BD82D8759CE4DC42C0D40C49C0|yceB], (37,4%)
Expression and Regulation
Operons
genes
[gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
description
[Pubmed|15668000]
regulation
induced in the presence of methionine and taurine [Pubmed|11390694]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16109943], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
[protein|741156D495BE3857683C8A0390764EAD83845ABC|ascR]: activation, [Pubmed|16109943], in [regulon|protein:741156D495BE3857683C8A0390764EAD83845ABC|ascR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15272571], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A174 (ytmO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/174 NBRP B. subtilis, Japan]
BKE29330 (Δ[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGGTCTAAAATACTTAAGC, downstream forward: _UP4_AAACGTTAAGGAGGGACAGA
BKK29330 (Δ[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGGTCTAAAATACTTAAGC, downstream forward: _UP4_AAACGTTAAGGAGGGACAGA
References
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Time of last update: 2024-04-18 16:20:21
Author of last update: Jstuelk