khpA

khpA
168

putative RNA chaperone

locus
BSU_16000
Molecular weight
8.93 kDa
pI
7.34
Protein length
Gene length
function
unknown
product
putative RNA chaperone
essential
no
ec
null
synonyms
ylqC, KhpA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1837 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,673,892  1,674,137
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The protein
Protein family
UPF0109 family (single member, according to UniProt)
[wiki|Domains]
[wiki|KH domain] (aa 34-81) (according to UniProt)
Structure
[AF|O31738]
Expression and Regulation
Operons
genes
[gene|8F559BFC80FE56EA0389A2336F4E9E6908562BCB|ylxM]-[gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh]-[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]
description
[pubmed|36840557]
regulation
the mRNA is processed between [gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh] and [gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
expression
expression is decreased upon depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8335643], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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Array

2024-03-21 22:21:17

Jstuelk

26

0C22E9A74563E6DB840352CF68BC5D70BBD11EAB

0C22E9A74563E6DB840352CF68BC5D70BBD11EAB

genes
[gene|8F559BFC80FE56EA0389A2336F4E9E6908562BCB|ylxM]-[gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh]-[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]-[gene|0AB1FB44E8F28944B82B5EF14A9CDCFEE46DB76B|ylqD]-[gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]-[gene|885ECD9FAAC8BC95D32F049825D6B6244C046112|trmD]-[gene|51368512B8322EDBCA92674EC2B97CF26087D8A9|rplS]
description
[Pubmed|29794222]
regulation
the mRNA is processed between [gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh] and [gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
expression
expression is decreased upon depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8335643], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|8F559BFC80FE56EA0389A2336F4E9E6908562BCB|ylxM]→[gene|51368512B8322EDBCA92674EC2B97CF26087D8A9|rplS]

2024-02-07 15:26:05

Jstuelk

82

4F2FF9A3B937EE85312D0B0F9DA494E4B44E2297

417C7007C2492335257F8AEF606BB8627753757F

genes
[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]
description
[Pubmed|36840557,8335643]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
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[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]→[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]

2024-01-23 23:32:25

Jstuelk

67

EBA4231EDC32B79E8B88019E02F3880BAF1378CE

5C4CCA15D9411D61A7B6109A29C1A8986F928694

genes
[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]-[gene|0AB1FB44E8F28944B82B5EF14A9CDCFEE46DB76B|ylqD]-[gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]-[gene|885ECD9FAAC8BC95D32F049825D6B6244C046112|trmD]-[gene|51368512B8322EDBCA92674EC2B97CF26087D8A9|rplS]
description
[Pubmed|36840557]
Open in new tab

[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]→[gene|51368512B8322EDBCA92674EC2B97CF26087D8A9|rplS]

2024-03-07 02:42:46

Jstuelk

29

B00427AA69EF576CAC3B8B5C44347F99001CAF0D

B00427AA69EF576CAC3B8B5C44347F99001CAF0D

genes
[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]-[gene|0AB1FB44E8F28944B82B5EF14A9CDCFEE46DB76B|ylqD]-[gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]
description
[Pubmed|36840557]
Open in new tab

[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]→[gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]

2024-03-27 00:28:38

Jstuelk

24

B1D4FB4E8DDCF0CB0481B85ABEADC1254ACBB0DF

B1D4FB4E8DDCF0CB0481B85ABEADC1254ACBB0DF

Biological materials
Mutant
MGNA-B173 (ylqC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1172 NBRP B. subtilis, Japan]
1A827 (''ylqC''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A827&Search=1A827 BGSC]
BKE16000 ([gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCTTCCAAGTGTTGATCAG,  downstream forward: _UP4_TAAAAGGGAGAGGGCCGGCA
BKK16000 ([gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCTTCCAAGTGTTGATCAG,  downstream forward: _UP4_TAAAAGGGAGAGGGCCGGCA
Expression vectors
pGP3616 expression of [gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]-Strep by [wiki|pGP382] in B. subtilis suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
Reviews
34748246,36882621,38052429
References
8335643,36840557

67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599

Page visits: 1893

Time of last update: 2024-03-28 11:15:24

Author of last update: Jstuelk