rqcH
168
ribosome-associated protein quality control factor, selects tRNAAla during RQC elongation
locus
BSU_15640
Molecular weight
65.30 kDa
pI
8.98
function
rescue of stalled ribosomes
product
ribosome-associated protein quality control factor
essential
no
synonyms
rqcH, yloA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1293 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,636,131 1,637,849
Phenotypes of a mutant
defective in [wiki|biofilm formation] [pubmed|28294433]
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The protein
Catalyzed reaction/ biological activity
senses stalled translation intermediates and recruits tRNAAla(UGC) to modify nascent-chain C termini with a polyalanine degron for degradation [pubmed|34255840,33259811]
Protein family
NEMF family (single member, according to UniProt)
Structure
[PDB|8S1U] [protein|BD5ACF930DD63E258D71569326752C9A1D7B9324|ylmH] and [protein|003047E90159765F9F0E499FD760F95FFF4AC360|rqcH] bound to the 50S ribosome subunit [https://www.biorxiv.org/content/10.1101/2024.03.03.583159v1.full.pdf bioRxiv]
[AF|O34693]
Expression and Regulation
Operons
genes
[gene|003047E90159765F9F0E499FD760F95FFF4AC360|rqcH]
description
[pubmed|22383849]
regulation
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression during logarithmic growth [Pubmed|28294433]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: repression during stationary phase [Pubmed|28294433]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [pubmed|28294433], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: repression, [pubmed|28294433], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
Biological materials
Mutant
MGNA-B371 ([gene|search|yloA]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1370 NBRP B. subtilis, Japan]
BKE15640 ([gene|003047E90159765F9F0E499FD760F95FFF4AC360|rqcH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATACACCCTCTCATTCTT, downstream forward: _UP4_TGAGCCGCATAAAGAAAAGC
BKK15640 ([gene|003047E90159765F9F0E499FD760F95FFF4AC360|rqcH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATACACCCTCTCATTCTT, downstream forward: _UP4_TGAGCCGCATAAAGAAAAGC
References
Reviews
Original Publications
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Time of last update: 2024-04-17 18:54:13
Author of last update: Jstuelk