yisZ

yisZ
168

adenylylsulfate kinase

locus
BSU_10910
Molecular weight
22.16 kDa
pI
7.21
Protein length
Gene length
function
sulfate reduction and activation
product
adenylylsulfate kinase
essential
no
ec
2.7.1.25
synonyms
yisZ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0529 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,169,877 → 1,170,476
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The protein
Catalyzed reaction/ biological activity
adenosine 5'-phosphosulfate + ATP --> 3'-phosphoadenylyl sulfate + ADP + H+ (according to UniProt)
Protein family
APS kinase family (with [protein|3C71659E4868744A9301C26608EABF7B98217DCF|cysC], according to UniProt)
Structure
[PDB|3CR8] (from ''Escherichia coli'', 38% identity, 55% similarity) [Pubmed|19770499]
[AF|O06735]
Expression and Regulation
Operons
genes
[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]-[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]-[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]
description
[Pubmed|22383849]
regulation
expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,15383836]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
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[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]→[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]

2024-01-19 21:55:12

ghost

51

4fca3e0835090887f1ca8f30217e3a4a8ad039c1

C607370DEEFC425F067119B36C7AC0A95C3ADF71

Biological materials
Mutant
MGNA-A499 (yisZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/499 NBRP B. subtilis, Japan]
BKE10910 (Δ[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCAAATGATGTTCGGATTGT,  downstream forward: _UP4_TAAAAAACCCGATCAATGTC
BKK10910 (Δ[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCAAATGATGTTCGGATTGT,  downstream forward: _UP4_TAAAAAACCCGATCAATGTC
References
15699190,15383836

FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB

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Time of last update: 2024-03-28 03:53:25

Author of last update: Melvin.boenninger