ycgT
168
NADPH:ferredoxin oxidoreductase
locus
BSU_03270
Molecular weight
36.81 kDa
pI
5.61
function
unknown
product
NADPH:ferredoxin oxidoreductase
essential
no
synonyms
ycgT
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0492 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
352,858 → 353,868
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The protein
Catalyzed reaction/ biological activity
H+ + NADP+ + 2 reduced [2Fe-2S]-[ferredoxin] --> NADPH + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
Protein family
ferredoxin--NADP reductase type 2 family (with [protein|BAA74A6971CED9FB55540F1BC602CDA287D83E74|yumC], according to UniProt)
[wiki|Cofactors]
FAD [Pubmed|25826316]
Structure
[PDB|5XHU]
[AF|O31475]
Paralogous protein(s)
[protein|BAA74A6971CED9FB55540F1BC602CDA287D83E74|yumC]
Additional information
The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin
reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]
Expression and Regulation
Operons
genes
[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]
description
[pubmed|22383849]
regulation
induced under conditions of iron starvation ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|16672620]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [Pubmed|16672620], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
additional information
the mRNA is very stable (half-life > 15 min) [Pubmed|12884008]
Biological materials
Mutant
MGNA-C052 (ycgT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2050 NBRP B. subtilis, Japan]
GP1852 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::cat), available in [wiki|Jörg Stülke]'s lab
BKE03270 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
BKK03270 (Δ[gene|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|ycgT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
References
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Time of last update: 2024-04-19 01:03:55
Author of last update: Melvin.boenninger