SubtiBank SubtiBank

It will happen very soon:

International Conference on Bacilli and Gram-positive bacteria

June 11 - 15, 2017, Berlin, Germany

Check out the program here

Categories containing this gene/protein

carbon core metabolism, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

CggR regulon


  • Coordinates on the chromosome (coding sequence): 3,479,405 -> 3,480,166
  • Phenotypes of a mutant

  • essential PubMed, non-essential according to PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)
  • Protein family

  • triosephosphate isomerase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information



  • phosphorylation on Ser-213 PubMed
  • Cofactors

    Effectors of protein activity

  • inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed
  • Structure

  • 1BTM (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI
  • Localization

  • cytoplasm (homogeneously distributed throughout the cell) PubMed
  • Interactions

  • Tkt-Tpi PubMed
  • Additional information

  • extensive information on the structure and enzymatic properties of Tpi can be found at Proteopedia
  • Expression and Regulation


  • cggR-gapA-pgk-tpiA-pgm-eno PubMed
  • pgk-tpiA-pgm-eno PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • expression activated by glucose (3.3 fold) PubMed
  • cggR: induced by glycolytic substrates CggR PubMed
  • pgk: constitutive PubMed
  • Regulatory mechanism

  • transcription repression by CggR PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Biological materials


  • GP700 (cat), available in Jörg Stülke's lab, PubMed
  • Expression vector

  • pGP394 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • pGP89 (N-terminal Strep-tag, for SPINE, expression in B. subtilis), available in Jörg Stülke's lab
  • pGP1511 (expression in B. subtilis, in pBQ200), available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct


    Labs working on this gene/protein


    Monahan LG, Hajduk IV, Blaber SP, Charles IG, Harry EJ

    Coordinating bacterial cell division with nutrient availability: a role for glycolysis

    MBio. 2014 May 13;5(3):e00935-14. doi: 10.1128/mBio.00935-14. PubMed PMID: 24825009; PubMed Central PMCID: PMC4030479.
    Commichau FM, Pietack N, Stülke J

    Essential genes in Bacillus subtilis: a re-evaluation after ten years

    Mol Biosyst. 2013 Jun;9(6):1068-75. doi: 10.1039/c3mb25595f. Epub 2013 Feb 18. Review. PubMed PMID: 23420519.
    Brown CT, Fishwick LK, Chokshi BM, Cuff MA, Jackson JM 4th, Oglesby T, Rioux AT, Rodriguez E, Stupp GS, Trupp AH, Woollcombe-Clarke JS, Wright TN, Zaragoza WJ, Drew JC, Triplett EW, Nicholson WL

    Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation

    Appl Environ Microbiol. 2011 Oct;77(19):6867-77. doi: 10.1128/AEM.05272-11. Epub 2011 Aug 5. PubMed PMID: 21821766; PubMed Central PMCID: PMC3187084.
    Commichau FM, Rothe FM, Herzberg C, Wagner E, Hellwig D, Lehnik-Habrink M, Hammer E, Völker U, Stülke J

    Novel activities of glycolytic enzymes in Bacillus subtilis: interactions with essential proteins involved in mRNA processing

    Mol Cell Proteomics. 2009 Jun;8(6):1350-60. doi: 10.1074/mcp.M800546-MCP200. Epub 2009 Feb 3. PubMed PMID: 19193632; PubMed Central PMCID: PMC2690492.
    Jannière L, Canceill D, Suski C, Kanga S, Dalmais B, Lestini R, Monnier AF, Chapuis J, Bolotin A, Titok M, Le Chatelier E, Ehrlich SD

    Genetic evidence for a link between glycolysis and DNA replication

    PLoS One. 2007 May 16;2(5):e447. PubMed PMID: 17505547; PubMed Central PMCID: PMC1866360.
    Macek B, Mijakovic I, Olsen JV, Gnad F, Kumar C, Jensen PR, Mann M

    The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis

    Mol Cell Proteomics. 2007 Apr;6(4):697-707. Epub 2007 Jan 10. PubMed PMID: 17218307.
    Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M

    A comprehensive proteome map of growing Bacillus subtilis cells

    Proteomics. 2004 Oct;4(10):2849-76. PubMed PMID: 15378759.
    Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J

    Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways

    Metab Eng. 2003 Apr;5(2):133-49. PubMed PMID: 12850135.
    Ludwig H, Homuth G, Schmalisch M, Dyka FM, Hecker M, Stülke J

    Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon

    Mol Microbiol. 2001 Jul;41(2):409-22. PubMed PMID: 11489127.
    Delboni LF, Mande SC, Rentier-Delrue F, Mainfroid V, Turley S, Vellieux FM, Martial JA, Hol WG

    Crystal structure of recombinant triosephosphate isomerase from Bacillus stearothermophilus

    An analysis of potential thermostability factors in six isomerases with known three-dimensional structures points to the importance of hydrophobic interactions. Protein Sci. 1995 Dec;4(12):2594-604. PubMed PMID: 8580851; PubMed Central PMCID: PMC2143043.
    Leyva-Vazquez MA, Setlow P

    Cloning and nucleotide sequences of the genes encoding triose phosphate isomerase, phosphoglycerate mutase, and enolase from Bacillus subtilis

    J Bacteriol. 1994 Jul;176(13):3903-10. PubMed PMID: 8021172; PubMed Central PMCID: PMC205587.
    No login