tgt
168
tRNA-guanine transglycosylase
locus
BSU_27710
Molecular weight
43.55 kDa
pI
6.15
function
tRNA modification
product
tRNA-guanine transglycosylase
essential
no
ec
2.4.2.29
synonyms
tgt
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0343 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,832,727 2,833,872
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The protein
Catalyzed reaction/ biological activity
7-aminomethyl-7-carbaguanine + guanosine34 in tRNA --> 7-aminomethyl-7-carbaguanosine34 in tRNA + guanine (according to UniProt)
Protein family
queuine tRNA-ribosyltransferase family (single member, according to UniProt)
Structure
[PDB|1PUD] (from Zymomonas mobilis, 52% identity) [pubmed|8654383]
[AF|O32053]
[wiki|Localization]
cytosol(according to UniProt)
Expression and Regulation
Operons
genes
[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[gene|6CDF1836F5B613A6718308A4A27D713193EA197B|yrzS]-[gene|23174B2A19DEDF2F20858A005D0681D36E15E6E3|queA]-[gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]-[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]
description
[pubmed|22383849]
regulation
the mRNA is processed between [gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt] and [gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
Biological materials
Mutant
BKE27710 ([gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT, downstream forward: _UP4_TAAATAAACATAACAGGCTT
BKK27710 ([gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTGATGAGGCCTCCT, downstream forward: _UP4_TAAATAAACATAACAGGCTT
References
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Time of last update: 2024-04-19 14:39:58
Author of last update: Jstuelk