tagT
168
phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
locus
BSU_35840
Molecular weight
35.65 kDa
pI
9.55
function
transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
product
phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
essential
no
synonyms
tagT, ywtF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1316 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,694,239 3,695,207
Phenotypes of a mutant
a ''[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]'' mutant has no phenoype, the triple ''[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT] [gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU] [gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]'' mutant is unable to grow under normal conditions [Pubmed|21964069]
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The protein
Protein family
LytR/CpsA/Psr (LCP) family (with [protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU] and [protein|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV], according to UniProt) [Pubmed|19099556]
[wiki|Cofactors]
Mg2+ [Pubmed|29107701,21964069]
Structure
[PDB|6UF5] (aa 46-322) [pubmed|31969390]
[PDB|3MEJ]
[PDB|4DE9] [Pubmed|22432711]
[PDB|6MPS] (bound to LIIa-WTA] [Pubmed|29402087]
[PDB|6MPT] (bound to LI-WTA] [Pubmed|29402087]
[AF|Q7WY78]
Paralogous protein(s)
[protein|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV], [protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU]
[wiki|Localization]
cytoplasmic membrane (with extracytoplasmic catalaytic domain) [Pubmed|21964069]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]
description
[Pubmed|9353933]
regulation
expressed at high salt concentrations ([protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]) [pubmed|18179421]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
genes
[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]-[gene|9730F7C18AC7CA68D144717851A0C261D9647E31|ywtE]
description
[Pubmed|22383849]
regulation
expressed at high salt concentrations ([protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]) [pubmed|18179421]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
Biological materials
Mutant
MGNA-A547 (ywtF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/547 NBRP B. subtilis, Japan]
BKE35840 ([gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATCAATACCTCACGT, downstream forward: _UP4_TAATGAACGGGATGGCGATT
BKK35840 ([gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATCAATACCTCACGT, downstream forward: _UP4_TAATGAACGGGATGGCGATT
References
Reviews
Original Publications
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Time of last update: 2024-04-19 13:48:04
Author of last update: Jstuelk