spoVM
168
membrane curvature sensor, required for normal spore cortex and coat synthesis, inhibits the proteolytic activity of [protein|search|FtsH ](adaptor protein)
locus
BSU_15810
Molecular weight
2.88 kDa
pI
11.03
function
initiation of spore coat assembly
product
membrane curvature sensor
essential
no
synonyms
spoVM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5844 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,655,446 → 1,655,526
Phenotypes of a mutant
reduction of sporulation efficiency due to defects in spore coat and cortex assembly [Pubmed|22463703]
Advertisement
The protein
Catalyzed reaction/ biological activity
anchors the basement layer of the spore coat to the surface of the developing spore [Pubmed|22463703]
preferentially binds to micron-scale convex membranes and marks the surface of the developing spore as the site for coat assembly [pubmed|31597735]
recruits [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] to the spore coat [pubmed|31597735]
[wiki|Domains]
contains an amphipathic alpha-helix
Structure
[PDB|2MVH] [Pubmed|25825747]
[AF|P37817]
[wiki|Localization]
around the surface of the developing forespore [Pubmed|22463703]
spore membrane, recognizes membrane curvature [Pubmed|19265022]
spore coat (basement), localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] [Pubmed|22171814]
Expression and Regulation
Operons
genes
[gene|BBDA32A4EE3389D6F4404F7B3DA9E13AC7BF8055|spoVM]
description
[Pubmed|8231808]
regulation
expressed in mother cell during sporulation ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|8231808,15699190,19265022]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: activation, [Pubmed|8231808], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8231808,15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE15810 (Δ[gene|BBDA32A4EE3389D6F4404F7B3DA9E13AC7BF8055|spoVM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTGTCCCCTCCTATA, downstream forward: _UP4_TAATGCCCAGGGGTTCAAAG
BKK15810 (Δ[gene|BBDA32A4EE3389D6F4404F7B3DA9E13AC7BF8055|spoVM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTGTCCCCTCCTATA, downstream forward: _UP4_TAATGCCCAGGGGTTCAAAG
References
Reviews
Original Publications
Page visits: 2920
Time of last update: 2024-04-18 19:58:55
Author of last update: Jstuelk