spoIID

spoIID
168

cell wall hydrolase (lytic transglycosylase), required for complete dissolution of the asymmetric septum

locus
BSU_36750
Molecular weight
37.25 kDa
pI
9.76
Protein length
Gene length
function
dissolution of the septal cell wall
product
lytic transglycosylase
essential
no
synonyms
spoIID, spoIIC

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2385 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,776,722  3,777,753
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The protein
Catalyzed reaction/ biological activity
degrades the glycan strands of the peptidoglycan into disaccharide units [Pubmed|20159959], enhances [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] activity [Pubmed|20159959]
required for proper localization of [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] (together with [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]) [Pubmed|23834622]
Structure
[PDB|4RWR] (from ''B. anthracis'', 54% identity, 74% similarity) [pubmed|27226615]
[AF|P07372]
[wiki|Localization]
cell membrane [Pubmed|20159959]
the complex of [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID], [protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM], and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] localizes at the leading edge of the mother cell engulfing membrane and is essential and rate limiting for membrane migration [Pubmed|20382772,12502745]
localization of the [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]-[protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]-[protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] complex to the septal membrane depends on [protein|D98D61CA651AAA415386B1B2B41562E5CF77DEF1|spoIIB] [pubmed|36066101]
Expression and Regulation
Operons
genes
[gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]
description
[Pubmed|3011962]
regulation
expressed early during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE], [wiki|SpoIIID]) [Pubmed|15699190,15383836]
repressed by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P binding to a box downstream of the promoter [pubmed|37485800]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [pubmed|37485800], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
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[gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]

2024-03-13 23:00:14

Jstuelk

48

74579E7C6A1758BEACE5076807AF9FFACC2488F2

D2B66FFEDDFD13CC013E69FA404F5C13EF03D405

Biological materials
Mutant
BKE36750 ([gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCTGCCTCCTGC,  downstream forward: _UP4_TAGAAAAAGACGCTCATTGG
BKK36750 ([gene|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCAGCTGCCTCCTGC,  downstream forward: _UP4_TAGAAAAAGACGCTCATTGG
References
Reviews
23944268,32660383,33651833,35638784
Original Publications
17376078,1946462,11886548,15752199,3011962,15699190,15383836,20159959,20382772,12502745,20444098,23834622,23859254,27226615,31282858,36066101,37485800

AAC4BF6FA80115AB90D2B161C1D5383625953616

Page visits: 3474

Time of last update: 2024-03-28 17:25:32

Author of last update: Jstuelk