SubtiBank SubtiBank

Categories containing this gene/protein


utilization of specific carbon sources

This gene is a member of the following regulons


DegU regulon, SacY regulon

Gene


  • Coordinates on the chromosome (coding sequence): 3,536,012 -> 3,537,433
  • The protein


    Catalyzed reaction/ biological activity

  • Sucrose (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose (6)-beta-D-fructofuranosyl-(2->)(n 1) alpha-D-glucopyranoside (according to Swiss-Prot)
  • Protein family

  • glycosyl hydrolase 68 family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 1PT2 (complex with sucrose), 1OYG
  • Localization

  • secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed
  • Interactions

    Additional information

    Expression and Regulation


    Operon

  • sacB-levB-yveA PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • induction by sucrose (at high concentration) (SacY) PubMed
  • Regulatory mechanism

  • SacY: binding to a RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed
  • Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

  • pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Stülke lab
  • GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Marvasi M(1), Visscher PT, Casillas Martinez L.

    Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.

    FEMS Microbiol Lett. 2010 Dec;313(1):1-9. doi: 10.1111/j.1574-6968.2010.02085.x.DOI: 10.1111/j.1574-6968.2010.02085.x PMID: 20735481 [Indexed for MEDLINE]

    Original publications

    Raga-Carbajal E, Carrillo-Nava E, Costas M, Porras-Dominguez J, López-Munguía A, Olvera C

    Size product modulation by enzyme concentration reveals two distinct levan elongation mechanisms in Bacillus subtilis levansucrase

    Glycobiology. 2016 Apr;26(4):377-85. doi: 10.1093/glycob/cwv112. PubMed PMID: 26646447.
    Méndez-Lorenzo L, Porras-Domínguez JR, Raga-Carbajal E, Olvera C, Rodríguez-Alegría ME, Carrillo-Nava E, Costas M, López Munguía A

    Intrinsic Levanase Activity of Bacillus subtilis 168 Levansucrase (SacB)

    PLoS One. 2015 Nov 23;10(11):e0143394. doi: 10.1371/journal.pone.0143394. PubMed PMID: 26600431; PubMed Central PMCID: PMC4658133.
    Porras-Domínguez JR, Ávila-Fernández Á, Miranda-Molina A, Rodríguez-Alegría ME, Munguía AL

    Bacillus subtilis 168 levansucrase (SacB) activity affects average levan molecular weight

    Carbohydr Polym. 2015 Nov 5;132:338-44. doi: 10.1016/j.carbpol.2015.06.056. PubMed PMID: 26256357.
    Olvera C, Centeno-Leija S, Ruiz-Leyva P, López-Munguía A

    Design of chimeric levansucrases with improved transglycosylation activity

    Appl Environ Microbiol. 2012 Mar;78(6):1820-5. doi: 10.1128/AEM.07222-11. PubMed PMID: 22247149; PubMed Central PMCID: PMC3298123.
    Voigt B, Antelmann H, Albrecht D, Ehrenreich A, Maurer KH, Evers S, Gottschalk G, van Dijl JM, Schweder T, Hecker M

    Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis

    J Mol Microbiol Biotechnol. 2009;16(1-2):53-68. doi: 10.1159/000142894. PubMed PMID: 18957862.
    Tsukahara K, Ogura M

    Promoter selectivity of the Bacillus subtilis response regulator DegU, a positive regulator of the fla/che operon and sacB

    BMC Microbiol. 2008 Jan 15;8:8. doi: 10.1186/1471-2180-8-8. PubMed PMID: 18197985; PubMed Central PMCID: PMC2245950.
    Pereira Y, Petit-Glatron MF, Chambert R

    yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis

    Microbiology. 2001 Dec;147(Pt 12):3413-9. PubMed PMID: 11739774.
    Gay P, Le Coq D, Steinmetz M, Ferrari E, Hoch JA

    Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli

    J Bacteriol. 1983 Mar;153(3):1424-31. PubMed PMID: 6402497; PubMed Central PMCID: PMC221793.
    Aymerich S, Steinmetz M

    Cloning and preliminary characterization of the sacS locus from Bacillus subtilis which controls the regulation of the exoenzyme levansucrase

    Mol Gen Genet. 1987 Jun;208(1-2):114-20. PubMed PMID: 3039303.
    Shimotsu H, Henner DJ

    Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes

    J Bacteriol. 1986 Oct;168(1):380-8. PubMed PMID: 2428811; PubMed Central PMCID: PMC213462.
    Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D

    Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system

    J Bacteriol. 1990 Feb;172(2):1043-50. PubMed PMID: 2105292; PubMed Central PMCID: PMC208535.
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