rocE
168
amino acid permease
locus
BSU_40330
Molecular weight
51.47 kDa
pI
8.53
function
arginine, ornithine and citrulline uptake
product
amino acid permease
essential
no
synonyms
rocE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0833 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,142,675 → 4,144,078
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The protein
Protein family
[wiki|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
Structure
[PDB|6F34] (from Geobacillus kaustophilus, 23% identity) [pubmed|29416041]
[AF|P39137]
Paralogous protein(s)
[protein|B6A920650919D5BBBD75CCA58C30A0D8ECE64DA3|ybgF], [protein|C7FEA1D9C7C96C39CEE83CE3F13D412DE6AFE10E|ydgF], [protein|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|aapA], [protein|1594BA83CED2DA9F86C1CE2A0DC6E1C9B665EACB|gabP], [protein|219586A3F378DC38EA076FD39B99D41136BDE722|alaP], [protein|423AEC9D260BE53AD22851D999244B89BEB3E84D|hutM], [protein|8C4A8961B2514A8AD531AFB104B10C0797D02AC2|yvbW], [protein|9AD0E43119BDC65D5F67CE55CD9417E1E6BAE058|aimB], [protein|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]
[wiki|Localization]
cell membrane (according to UniProt), membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]-[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]-[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]
description
[Pubmed|7540694]
regulation
induced in the presence of arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]) [Pubmed|7565595]
induced in the presence of arginine, citrulline or ornithine (with ornithine as the molecular inducer) ([protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]) [pubmed|37343703]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]: activation, [Pubmed|7540694], in [regulon|protein:62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]: activation, [Pubmed|7540694], in [regulon|protein:BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|7540694], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
additional information
expression of the [gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]-[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]-[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF] operon is increased upon depletion of [gene|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA] (resulting from increased [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] expression) [http://www.nature.com/articles/nmicrobiol20157 Reference]
Biological materials
Mutant
GP1690 Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::''tet'', available in [wiki|Jörg Stülke]'s lab
GP4156 (Δ[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]::''aphA3'', Δ[gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]::''ermC'', Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::''tet'',), available in [wiki|Jörg Stülke]'s lab
BKE40330 (Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATTCTGCACCCCTTCAG, downstream forward: _UP4_TAAAAACGTTGTCCGCAAGT
BKK40330 (Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATTCTGCACCCCTTCAG, downstream forward: _UP4_TAAAAACGTTGTCCGCAAGT
References
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Time of last update: 2024-04-18 22:37:04
Author of last update: Jstuelk