rho

rho
168

transcriptional termination protein, prevents the formation of antiterminator-like RNA structures that could otherwise compete with the terminator hairpin, involved in balanced regulation of cell motility, [category|SW.4.1.2|Biofilm formation], and [category|SW.4.2|Sporulation]

locus
BSU_37080
Molecular weight
48.51 kDa
pI
6.86
Protein length
427 aaSequenceBlast
Gene length
1284 bpSequenceBlast
function
transcriptional termination
product
transcriptional termination protein
essential
no
synonyms
rho
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1158 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,803,400  3,804,683
Phenotypes of a mutant
loss of motility due to poor expression of the [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD] regulon, this can be partially reversed by inactivation of [gene|920F91E748EE079FF864011D9052B073567C41E4|slrR] [pubmed|28723971]
no [category|SW.4.1.2|Biofilm formation], this can be suppressed by deletion of [gene|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] and by switching of expression of the [gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]-[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA] antisense transcript [pubmed|28723971]
increased efficiency of [category|SW.4.2|Sporulation], due to enhanced [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] phosphorylation [pubmed|28723971]
defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]
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The protein
Catalyzed reaction/ biological activity
prevents the formation of antiterminator-like RNA structures that could otherwise compete with the terminator hairpin [pubmed|36154805]
Protein family
Rho family (single member, according to UniProt)
[wiki|Domains]
[wiki|CSD domain] (aa 54-127) (according to the InterPro database)
Rho RNA-BD domain (aa 51-125) (according to UniProt)
Structure
[PDB|1PVO] (Rho from ''E. coli'') [Pubmed|12859904]
[PDB|8HSR] ([protein|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[wiki|RNA polymerase] termination complex from Thermus thermophilus) [pubmed|36753546]
[AF|Q03222]
Expression and Regulation
Operons
genes
[gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]-[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]
description
[pubmed|22383849]
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[gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]→[gene|035154A4160F084BA1CC124D0951937B203E167D|rpmE]

2024-04-16 22:03:25

Jstuelk

82

64fa041f2672c7788b782f58b6b0099d088adcfa

FCFBD42F1FD2067C74E71BD492FF864F6BD1C74A

Biological materials
Mutant
GP2673 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::''ermC''), available in [wiki|Jörg Stülke]'s lab
BKE37080 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACACCACGCTTTT,  downstream forward: _UP4_TAAATTGTATTTGCAAAAGA
BKK37080 ([gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACACCACGCTTTT,  downstream forward: _UP4_TAAATTGTATTTGCAAAAGA
References
Reviews
16946247,12887917,16946247,8576105,23347833,12887917,23704790,26796109,28731845,29094196,36154805
Original Publications
36815589,11566991,10027981,20075920,22383849,12859904,21040729,25112476,28723971,26857544,29931073,30845912,33243850,34805793,35638827,36192538,36735730,36753546

7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5

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Time of last update: 2024-04-16 15:35:00

Author of last update: Jstuelk