queD

queD
168

6-pyruvoyltetrahydropterin synthase, synthesis of the modified ribonucleotide queuosine

locus
BSU_13730
Molecular weight
16.71 kDa
pI
5.94
Protein length
149 aaSequenceBlast
Gene length
450 bpSequenceBlast
function
tRNA modification
product
6-pyruvoyltetrahydropterin synthase
essential
no
synonyms
queD, ykvK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0720 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,440,100 → 1,440,549
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The protein
Catalyzed reaction/ biological activity
7,8-dihydroneopterin 3'-triphosphate + H2O --> 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H+ + triphosphate (according to UniProt)
Protein family
PTPS family (single member, according to UniProt)
Structure
[PDB|3QN0] (from E. coli, 33% identity) [pubmed|24816091]
[AF|O31676]
Expression and Regulation
Operons
genes
[gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]
description
[Pubmed|14660578]
regulation
repressed in the presence of queuosine ([wiki|preQ1 riboswitch]) [Pubmed|19285444]
regulatory mechanism
preQ1 riboswitch: RNA switch, in the absence of queuosine [Pubmed|19285444], in [regulon|other_regulator:preQ1 riboswitch|preQ1 riboswitch]
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[gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]→[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]

2024-04-06 17:00:55

ghost

71

aa63ca74d3449d36e3337e94aa3ada3fd88e36c1

1D214C8F3DFBDBD36FAB40A469193DA1EEEACDF0

Biological materials
Mutant
MGNA-B325 (ykvK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1324 NBRP B. subtilis, Japan]
BKE13730 (Δ[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTTATGCACGCTCTCCTTT,  downstream forward: _UP4_TGAATGGCTAAAGGAATTCC
BKK13730 (Δ[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTTATGCACGCTCTCCTTT,  downstream forward: _UP4_TGAATGGCTAAAGGAATTCC
References
16411777,18259064,14660578,19285444,17384645,24816091

BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52

Page visits: 1981

Time of last update: 2024-04-17 18:23:44

Author of last update: Melvin.boenninger