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Categories containing this gene/protein


phosphotransferase systems, phosphoproteins, most abundant proteins

This gene is a member of the following regulons


GlcT regulon, stringent response

Gene


  • Coordinates on the chromosome (coding sequence): 1,459,650 -> 1,461,362
  • The protein


    Catalyzed reaction/ biological activity

  • Phosphoenolpyruvate protein L-histidine = pyruvate protein N(pi)-phospho-L-histidine (according to Swiss-Prot) PEP-dependent autophosphorylation on His-189, transfer of the phosphoryl group to HPr (His-15)
  • Protein family

  • PEP-utilizing enzyme family (according to Swiss-Prot) PEP-utilizing enzyme family
  • Paralogous protein(s)

    Kinetic information

    Domains

  • HPr binding site (N-Terminal Domain)
  • pyruvate binding site (C-Terminal Domain)
  • pyrophosphate/phosphate carrier histidine (central Domain)
  • Modification

  • transient autophosphorylation on His-189
  • in vivo also phosphorylated on Ser-34 or Ser-36 PubMed
  • Cofactors

  • Magnesium
  • Effectors of protein activity

    Structure

  • 2WQD (Enzyme I from Staphylococcus aureus, 68% identity) PubMed
  • Localization

  • cytoplasm, even distribution PubMed
  • Interactions

  • PtsI-PtsH
  • Additional information

    Expression and Regulation


    Operon

  • ptsG-ptsH-ptsI PubMed
  • ptsH-ptsI PubMed
  • Sigma factor

  • ptsG: SigA PubMed
  • ptsH: SigA PubMed
  • Regulation

  • expression activated by glucose (2 fold) (GlcT) PubMed
  • the ptsH promoter is constitutive PubMed
  • subject to negative stringent control upon amino acid limitation (due to control of ptsG transcription initiation) PubMed
  • Regulatory mechanism

  • ptsG: transcriptional antitermination via the GlcT-dependent RNA switch PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Biological materials


    Mutant

  • available in Jörg Stülke's lab:
  • GP864 (ermC)
  • GP778 (replacement of glcT and the ptsG-ptsH-ptsI operon by a spc cassette), PubMed
  • Expression vector

  • pAG3 (His-tag) PubMed, available in Galinier lab
  • for expression, purification in E. coli (His-tag), in pWH844: pGP813 available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Josef Deutscher, Paris-Grignon, France
  • Jörg Stülke, University of Göttingen, Germany Homepage
  • References


    Govindarajan S, Elisha Y, Nevo-Dinur K, Amster-Choder O

    The general phosphotransferase system proteins localize to sites of strong negative curvature in bacterial cells

    MBio. 2013 Oct 15;4(5):e00443-13. doi: 10.1128/mBio.00443-13. PubMed PMID: 24129255; PubMed Central PMCID: PMC3812706.
    Rothe FM, Wrede C, Lehnik-Habrink M, Görke B, Stülke J

    Dynamic localization of a transcription factor in Bacillus subtilis: the LicT antiterminator relocalizes in response to inducer availability

    J Bacteriol. 2013 May;195(10):2146-54. doi: 10.1128/JB.00117-13. Epub 2013 Mar 8. PubMed PMID: 23475962; PubMed Central PMCID: PMC3650534.
    Tojo S, Kumamoto K, Hirooka K, Fujita Y

    Heavy involvement of stringent transcription control depending on the adenine or guanine species of the transcription initiation site in glucose and pyruvate metabolism in Bacillus subtilis

    J Bacteriol. 2010 Mar;192(6):1573-85. doi: 10.1128/JB.01394-09. Epub 2010 Jan 15. PubMed PMID: 20081037; PubMed Central PMCID: PMC2832531.
    Oberholzer AE, Schneider P, Siebold C, Baumann U, Erni B

    Crystal structure of enzyme I of the phosphoenolpyruvate sugar phosphotransferase system in the dephosphorylated state

    J Biol Chem. 2009 Nov 27;284(48):33169-76. doi: 10.1074/jbc.M109.057612. Epub 2009 Sep 28. PubMed PMID: 19801641; PubMed Central PMCID: PMC2785159.
    Macek B, Mijakovic I, Olsen JV, Gnad F, Kumar C, Jensen PR, Mann M

    The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis

    Mol Cell Proteomics. 2007 Apr;6(4):697-707. Epub 2007 Jan 10. PubMed PMID: 17218307.
    Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M

    A comprehensive proteome map of growing Bacillus subtilis cells

    Proteomics. 2004 Oct;4(10):2849-76. PubMed PMID: 15378759.
    Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J

    Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways

    Metab Eng. 2003 Apr;5(2):133-49. PubMed PMID: 12850135.
    Garrity LF, Schiel SL, Merrill R, Reizer J, Saier MH Jr, Ordal GW

    Unique regulation of carbohydrate chemotaxis in Bacillus subtilis by the phosphoenolpyruvate-dependent phosphotransferase system and the methyl-accepting chemotaxis protein McpC

    J Bacteriol. 1998 Sep;180(17):4475-80. PubMed PMID: 9721285; PubMed Central PMCID: PMC107457.
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