proG

proG
168

1-pyrroline-5-carboxylate dehydrogenase

locus
BSU_12910
Molecular weight
30.05 kDa
pI
4.91
Protein length
Gene length
function
biosynthesis of proline
product
1-pyrroline-5-carboxylate dehydrogenase
essential
no
ec
1.5.1.2
synonyms
proG, ykeA, yzcA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0345 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,359,454  1,360,272
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The protein
Catalyzed reaction/ biological activity
1-pyrroline-5-carboxylate + NADPH + H+ --> L-proline + NADP+ [pubmed|28824574]
L-proline + NADP+ --> 1-pyrroline-5-carboxylate + 2 H+ + NADPH (according to UniProt)
L-proline + NAD+ --> 1-pyrroline-5-carboxylate + 2 H+ + NADH (according to UniProt)
Protein family
[wiki|pyrroline-5-carboxylate reductase family] [pubmed|28824574]
[wiki|Cofactors]
NADPH [pubmed|28824574]
Structure
[AF|Q00777]
Effectors of protein activity
feedback-inhibited by proline [pubmed|28824574]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]
description
[Pubmed|1766370,21784929]
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[gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]

2024-03-26 16:47:23

ghost

53

9563c62cb2cad4c9ca6a9ab4e852ff9e03d4e54e

B0D5771A0099742E0C49412813CA2447B0B2EC65

Biological materials
Mutant
MGNA-B308 (ykeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1307 NBRP B. subtilis, Japan]
BKE12910 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT,  downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
BKK12910 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT,  downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
GP3728 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::tet trpC2) available in the [wiki|Stülke] lab
GP3741 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::aphA3 trpC2) available in the [wiki|Stülke] lab
Expression vectors
pGP3791: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab
pGP3863: expression of Strep-''proG'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
References
Reviews
25367752
Original Publications
11418582,1766370,21784929,28752945,28824574

63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E

Page visits: 1401

Time of last update: 2024-03-29 07:55:09

Author of last update: Robert.warneke