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Categories containing this gene/protein


carbon core metabolism, essential genes, phosphoproteins

This gene is a member of the following regulons


CggR regulon

Gene


  • Coordinates on the chromosome (coding sequence): 3,477,877 -> 3,479,412
  • Phenotypes of a mutant

  • Essential PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • 2-phospho-D-glycerate = 3-phospho-D-glycerate (according to Swiss-Prot)
  • Protein family

  • BPG-independent phosphoglycerate mutase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

  • Reversible Michaelis-Menten PubMed
  • Domains

    Modification

  • phosphorylation on Ser-62 PubMed, PubMed
  • Cofactors

  • Mn2+
  • Effectors of protein activity

  • Inhibited by diverse divalent heavy-metal ions, EDTA and 2,3-butanedione PubMed
  • 2,3-Diphosphoglycerate has NO role on this enzyme regulation PubMed
  • Structure

  • 1EJJ (Geobacillus stearothermophilus, complex with 3-phosphoglycerate), 1EQJ (Geobacillus stearothermophilus, complex with 2-phosphoglycerate), Geobacillus stearothermophilus, complex with 2-phosphoglycerate NCBI, Geobacillus stearothermophilus, complex with 3-phosphoglycerate NCBI
  • Localization

  • Cytoplasm (Homogeneous) PubMed
  • Interactions

  • Pgm-PfkA
  • Additional information

  • extensive information on the structure and enzymatic properties of Pgm can be found at Proteopedia
  • Expression and Regulation


    Operon

  • cggR-gapA-pgk-tpiA-pgm-eno PubMed
  • pgk-tpiA-pgm-eno PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • expression activated by glucose (3.3 fold) PubMed
  • cggR: induced by glycolytic substrates CggR PubMed
  • pgk: constitutive PubMed
  • Regulatory mechanism

  • transcription repression by CggR PubMed
  • Additional information

    Biological materials


    Mutant

  • GP593 (pgm::cat), available in Jörg Stülke's lab, PubMed
  • GP598 (pgm::erm), available in Jörg Stülke's lab, PubMed
  • GP698 (pgm-eno::cat), available in Jörg Stülke's lab, PubMed
  • Expression vector

  • pGP1425 (expression of pgm in B. subtilis, in pBQ200), available in Jörg Stülke's lab
  • pGP1500 (expression of pgm and eno in B. subtilis, in pBQ200), available in Jörg Stülke's lab
  • pGP1101 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • pGP396 (Pgm-S62A, N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • pGP92 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Jörg Stülke, University of Göttingen, Germany Homepage
  • [Jedrzejas], Research Center Oakland, CA, USA Homepage
  • References


    Commichau FM, Pietack N, Stülke J

    Essential genes in Bacillus subtilis: a re-evaluation after ten years

    Mol Biosyst. 2013 Jun;9(6):1068-75. doi: 10.1039/c3mb25595f. Epub 2013 Feb 18. Review. PubMed PMID: 23420519.
    Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M

    Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis

    Proteomics. 2007 Oct;7(19):3509-26. PubMed PMID: 17726680.
    Jannière L, Canceill D, Suski C, Kanga S, Dalmais B, Lestini R, Monnier AF, Chapuis J, Bolotin A, Titok M, Le Chatelier E, Ehrlich SD

    Genetic evidence for a link between glycolysis and DNA replication

    PLoS One. 2007 May 16;2(5):e447. PubMed PMID: 17505547; PubMed Central PMCID: PMC1866360.
    Macek B, Mijakovic I, Olsen JV, Gnad F, Kumar C, Jensen PR, Mann M

    The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis

    Mol Cell Proteomics. 2007 Apr;6(4):697-707. Epub 2007 Jan 10. PubMed PMID: 17218307.
    Nukui M, Mello LV, Littlejohn JE, Setlow B, Setlow P, Kim K, Leighton T, Jedrzejas MJ

    Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase: a crucial enzyme for spores and growing cells of Bacillus species

    Biophys J. 2007 Feb 1;92(3):977-88. Epub 2006 Nov 3. PubMed PMID: 17085493; PubMed Central PMCID: PMC1779985.
    Nukui M, Mello LV, Littlejohn JE, Setlow B, Setlow P, Kim K, Leighton T, Jedrzejas MJ

    Structure and molecular mechanism of Bacillus anthracis cofactor-independent phosphoglycerate mutase: a crucial enzyme for spores and growing cells of Bacillus species

    Biophys J. 2007 Feb 1;92(3):977-88. Epub 2006 Nov 3. PubMed PMID: 17085493; PubMed Central PMCID: PMC1779985.
    Meile JC, Wu LJ, Ehrlich SD, Errington J, Noirot P

    Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory

    Proteomics. 2006 Apr;6(7):2135-46. PubMed PMID: 16479537.
    Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J

    Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways

    Metab Eng. 2003 Apr;5(2):133-49. PubMed PMID: 12850135.
    Rigden DJ, Lamani E, Mello LV, Littlejohn JE, Jedrzejas MJ

    Insights into the catalytic mechanism of cofactor-independent phosphoglycerate mutase from X-ray crystallography, simulated dynamics and molecular modeling

    J Mol Biol. 2003 May 9;328(4):909-20. PubMed PMID: 12729763.
    Rigden DJ, Mello LV, Setlow P, Jedrzejas MJ

    Structure and mechanism of action of a cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus with broad specificity phosphatase activity

    J Mol Biol. 2002 Feb 1;315(5):1129-43. PubMed PMID: 11827481.
    Jedrzejas MJ, Setlow P

    Comparison of the binuclear metalloenzymes diphosphoglycerate-independent phosphoglycerate mutase and alkaline phosphatase: their mechanism of catalysis via a phosphoserine intermediate

    Chem Rev. 2001 Mar;101(3):607-18. Review. PubMed PMID: 11712498.
    Jedrzejas MJ, Setlow P

    Comparison of the binuclear metalloenzymes diphosphoglycerate-independent phosphoglycerate mutase and alkaline phosphatase: their mechanism of catalysis via a phosphoserine intermediate

    Chem Rev. 2001 Mar;101(3):607-18. Review. PubMed PMID: 11712498.
    Rigden DJ, Bagyan I, Lamani E, Setlow P, Jedrzejas MJ

    A cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus is actually a broad specificity phosphatase

    Protein Sci. 2001 Sep;10(9):1835-46. PubMed PMID: 11514674; PubMed Central PMCID: PMC2253200.
    Ludwig H, Homuth G, Schmalisch M, Dyka FM, Hecker M, Stülke J

    Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon

    Mol Microbiol. 2001 Jul;41(2):409-22. PubMed PMID: 11489127.
    Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G

    Mechanism of catalysis of the cofactor-independent phosphoglycerate mutase from Bacillus stearothermophilus

    Crystal structure of the complex with 2-phosphoglycerate. J Biol Chem. 2000 Jul 28;275(30):23146-53. PubMed PMID: 10764795.
    Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G

    Mechanism of catalysis of the cofactor-independent phosphoglycerate mutase from Bacillus stearothermophilus

    Crystal structure of the complex with 2-phosphoglycerate. J Biol Chem. 2000 Jul 28;275(30):23146-53. PubMed PMID: 10764795.
    Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G

    Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus

    EMBO J. 2000 Apr 3;19(7):1419-31. PubMed PMID: 10747010; PubMed Central PMCID: PMC313928.
    Jedrzejas MJ, Chander M, Setlow P, Krishnasamy G

    Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus

    EMBO J. 2000 Apr 3;19(7):1419-31. PubMed PMID: 10747010; PubMed Central PMCID: PMC313928.
    Chander M, Setlow P, Lamani E, Jedrzejas MJ

    Structural studies on a 2,3-diphosphoglycerate independent phosphoglycerate mutase from Bacillus stearothermophilus

    J Struct Biol. 1999 Jun 15;126(2):156-65. PubMed PMID: 10388626.
    Chander M, Setlow B, Setlow P

    The enzymatic activity of phosphoglycerate mutase from gram-positive endospore-forming bacteria requires Mn2+ and is pH sensitive

    Can J Microbiol. 1998 Aug;44(8):759-67. PubMed PMID: 9830105.
    Magill NG, Cowan AE, Leyva-Vazquez MA, Brown M, Koppel DE, Setlow P

    Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus species

    J Bacteriol. 1996 Apr;178(8):2204-10. PubMed PMID: 8636019; PubMed Central PMCID: PMC177926.
    Kuhn NJ, Setlow B, Setlow P

    Manganese(II) activation of 3-phosphoglycerate mutase of Bacillus megaterium: pH-sensitive interconversion of active and inactive forms

    Arch Biochem Biophys. 1993 Nov 1;306(2):342-9. PubMed PMID: 8215434.
    Leyva-Vazquez MA, Setlow P

    Cloning and nucleotide sequences of the genes encoding triose phosphate isomerase, phosphoglycerate mutase, and enolase from Bacillus subtilis

    J Bacteriol. 1994 Jul;176(13):3903-10. PubMed PMID: 8021172; PubMed Central PMCID: PMC205587.
    Watabe K, Freese E

    Purification and properties of the manganese-dependent phosphoglycerate mutase of Bacillus subtilis

    J Bacteriol. 1979 Feb;137(2):773-8. PubMed PMID: 33963; PubMed Central PMCID: PMC218356.
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