SubtiBank SubtiBank

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Categories containing this gene/protein


ATP synthesis, carbon core metabolism, phosphoproteins, universally conserved proteins, most abundant proteins

This gene is a member of the following regulons


CggR regulon

Gene


  • Coordinates on the chromosome (coding sequence): 3,480,197 -> 3,481,381
  • Phenotypes of a mutant

  • essential PubMed, non-essential according to PubMed
  • suppression of ftsZ(ts) mutation (reverted by addition of pyruvate) PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • ATP 3-phospho-D-glycerate = ADP 1,3-bisphosphoglycerate (according to Swiss-Prot)
  • Protein family

  • phosphoglycerate kinase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

  • Two Substrate Reversible Michaelis-Menten PubMed
  • Domains

  • nucleotide binding domain (ATP) (350–353)
  • 2x substrate binding domain (21–23), (59–62)
  • Modification

  • phosphorylation on Ser-183 AND Thr-299 PubMed, PubMed
  • Cofactors

  • Mg2 or Mn2 PubMed
  • Effectors of protein activity

  • Inhibited by Co2 , NDP and NMP PubMed
  • Structure

  • 1PHP (from Geobacillus stearothermophilus)
  • Localization

    cytoplasm (homogeneously distributed throughout the cell) PubMed

    Interactions

    Additional information

  • extensive information on the structure and enzymatic properties of Pgk can be found at Proteopedia
  • Expression and Regulation


    Operon

  • cggR-gapA-pgk-tpiA-pgm-eno PubMed
  • pgk-tpiA-pgm-eno PubMed
  • Sigma factor

  • cggR: SigA PubMed
  • pgk: SigA PubMed
  • Regulation

  • expression activated by glucose (3.3 fold) PubMed
  • cggR: induced by glycolytic substrates CggR PubMed
  • pgk: constitutive PubMed
  • Regulatory mechanism

  • transcription repression by CggR PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Biological materials


    Mutant

  • GP699 (pgk::cat), available in Jörg Stülke's lab, PubMedlab
  • GP707 (pgk::erm), available in Jörg Stülke's lab, PubMed
  • Expression vector

  • pGP1102 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • pGP95 (N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab
  • pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Jörg Stülke's lab
  • pGP1513 (expression in B. subtilis, in pBQ200), available in Jörg Stülke's lab
  • lacZ fusion

  • pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Jörg Stülke's lab
  • GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available iin Jörg Stülke's lab
  • FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Monahan LG, Hajduk IV, Blaber SP, Charles IG, Harry EJ

    Coordinating bacterial cell division with nutrient availability: a role for glycolysis

    MBio. 2014 May 13;5(3):e00935-14. doi: 10.1128/mBio.00935-14. PubMed PMID: 24825009; PubMed Central PMCID: PMC4030479.
    Commichau FM, Pietack N, Stülke J

    Essential genes in Bacillus subtilis: a re-evaluation after ten years

    Mol Biosyst. 2013 Jun;9(6):1068-75. doi: 10.1039/c3mb25595f. Epub 2013 Feb 18. Review. PubMed PMID: 23420519.
    Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M

    Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis

    Proteomics. 2007 Oct;7(19):3509-26. PubMed PMID: 17726680.
    Jannière L, Canceill D, Suski C, Kanga S, Dalmais B, Lestini R, Monnier AF, Chapuis J, Bolotin A, Titok M, Le Chatelier E, Ehrlich SD

    Genetic evidence for a link between glycolysis and DNA replication

    PLoS One. 2007 May 16;2(5):e447. PubMed PMID: 17505547; PubMed Central PMCID: PMC1866360.
    Macek B, Mijakovic I, Olsen JV, Gnad F, Kumar C, Jensen PR, Mann M

    The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis

    Mol Cell Proteomics. 2007 Apr;6(4):697-707. Epub 2007 Jan 10. PubMed PMID: 17218307.
    Meile JC, Wu LJ, Ehrlich SD, Errington J, Noirot P

    Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory

    Proteomics. 2006 Apr;6(7):2135-46. PubMed PMID: 16479537.
    Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M

    A comprehensive proteome map of growing Bacillus subtilis cells

    Proteomics. 2004 Oct;4(10):2849-76. PubMed PMID: 15378759.
    Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J

    Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways

    Metab Eng. 2003 Apr;5(2):133-49. PubMed PMID: 12850135.
    Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai K, Ashikaga S, Aymerich S, Bessieres P, Boland F, Brignell SC, Bron S, Bunai K, Chapuis J, Christiansen LC, Danchin A, Débarbouille M, Dervyn E, Deuerling E, Devine K, Devine SK, Dreesen O, Errington J, Fillinger S, Foster SJ, Fujita Y, Galizzi A, Gardan R, Eschevins C, Fukushima T, Haga K, Harwood CR, Hecker M, Hosoya D, Hullo MF, Kakeshita H, Karamata D, Kasahara Y, Kawamura F, Koga K, Koski P, Kuwana R, Imamura D, Ishimaru M, Ishikawa S, Ishio I, Le Coq D, Masson A, Mauël C, Meima R, Mellado RP, Moir A, Moriya S, Nagakawa E, Nanamiya H, Nakai S, Nygaard P, Ogura M, Ohanan T, O'Reilly M, O'Rourke M, Pragai Z, Pooley HM, Rapoport G, Rawlins JP, Rivas LA, Rivolta C, Sadaie A, Sadaie Y, Sarvas M, Sato T, Saxild HH, Scanlan E, Schumann W, Seegers JF, Sekiguchi J, Sekowska A, Séror SJ, Simon M, Stragier P, Studer R, Takamatsu H, Tanaka T, Takeuchi M, Thomaides HB, Vagner V, van Dijl JM, Watabe K, Wipat A, Yamamoto H, Yamamoto M, Yamamoto Y, Yamane K, Yata K, Yoshida K, Yoshikawa H, Zuber U, Ogasawara N

    Essential Bacillus subtilis genes

    Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4678-83. Epub 2003 Apr 7. PubMed PMID: 12682299; PubMed Central PMCID: PMC153615.
    Ludwig H, Homuth G, Schmalisch M, Dyka FM, Hecker M, Stülke J

    Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon

    Mol Microbiol. 2001 Jul;41(2):409-22. PubMed PMID: 11489127.
    Suzuki K, Imahori K

    Phosphoglycerate kinase from Bacillus stearothermophilus

    Methods Enzymol. 1982;90 Pt E:126-30. PubMed PMID: 7154941.
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