ohrR
168
transcription repressor of the [gene|869E46AECF6249610F8541E403D834284B62171D|ohrA] gene
locus
BSU_13150
Molecular weight
16.86 kDa
pI
6.36
function
regulation of [gene|869E46AECF6249610F8541E403D834284B62171D|ohrA] expression in response to organic peroxides
product
transcription repressor ([wiki|MarR family])
essential
no
synonyms
ohrR, ykmA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1846 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,381,434 1,381,877
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The protein
Protein family
[wiki|MarR family]
[wiki|Domains]
[wiki|HTH marR-type domain] (aa 11-141) (according to UniProt)
Structure
[PDB|1Z91] (reduced form), [PDB|1Z9C] (complex with ''[gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]'' promoter)
[AF|O34777]
Modification
senses organic peroxides and NaOCl, released from DNA upon oxidation of an active site cysteine
S-bacillithiolated by NaOCl and CHP stress on Cys-15, this results in release from the'' [gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]'' promoter [Pubmed|21749987]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|7A3A8AF7728474204396BB7A548F747D28E6FAC7|ohrR]
description
[Pubmed|9696771]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP3172 [gene|7A3A8AF7728474204396BB7A548F747D28E6FAC7|ohrR]::kan trpC2 available in [wiki|Jörg Stülke]'s lab
MGNA-A750 (ykmA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/750 NBRP B. subtilis, Japan]
BKE13150 ([gene|7A3A8AF7728474204396BB7A548F747D28E6FAC7|ohrR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCACCAACTCTT, downstream forward: _UP4_TGAGGATGCCTTGTACAGGC
BKK13150 ([gene|7A3A8AF7728474204396BB7A548F747D28E6FAC7|ohrR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTTCACCAACTCTT, downstream forward: _UP4_TGAGGATGCCTTGTACAGGC
labs
[wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
[wiki|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
References
Reviews
Original Publications
Page visits: 1635
Time of last update: 2024-04-16 02:16:11
Author of last update: Melvin.boenninger