lytD
168
peptidoglycan N-acetylglucosaminidase
locus
BSU_35780
Molecular weight
95.36 kDa
pI
9.94
function
major autolysin, cell separation
product
peptidoglycan N-acetylglucosaminidase
essential
no
ec
3.2.1.96
synonyms
lytD, cwlG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4193 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,684,826 3,687,468
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The protein
Catalyzed reaction/ biological activity
hydrolysis of the glycosidic bond between N-acetyl--d-glucosamine residues and adjacent monosaccharides [Pubmed|18266855]
Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact (according to UniProt)
Protein family
glycosyl hydrolase 73 family (with [protein|AF0BC3D534BC2CCCAAB53B286CF4286E58A8A338|lytG], according to UniProt)
[wiki|Domains]
3 SPOR domains (aa 70-149, aa 150-229, aa 230-311) (according to UniProt)
[wiki|SH3B domain] (aa 630-700) (according to UniProt)
Structure
[AF|P39848]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|29219315D31BA4ADE41687EFF17FE41D6C23D157|lytD]
description
[Pubmed|7934877]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|7934877], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Biological materials
Mutant
GP3400 (Δ[gene|29219315D31BA4ADE41687EFF17FE41D6C23D157|lytD]::neo trpC2), available in [wiki|Jörg Stülke]'s lab
1A788 ( ''lytD''::''tet''), [Pubmed|7934877], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A788&Search=1A788 BGSC]
1A792 ( ''lytD''::''tet''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]
BKE35780 ([gene|29219315D31BA4ADE41687EFF17FE41D6C23D157|lytD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTTCTCCTCTTTCTT, downstream forward: _UP4_TAAAAAACTTAGAAAGTTGC
BKK35780 ([gene|29219315D31BA4ADE41687EFF17FE41D6C23D157|lytD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCTTCTCCTCTTTCTT, downstream forward: _UP4_TAAAAAACTTAGAAAGTTGC
FLAG-tag construct
GP4405 ''lytD-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-04-20 09:21:58
Author of last update: Robert.warneke