lepA

lepA
168

elongation factor 4, GTP-binding protein

locus
BSU_25510
Molecular weight
68.41 kDa
pI
4.98
Protein length
612 aaSequenceBlast
Gene length
1839 bpSequenceBlast
function
translation elongation
product
elongation factor 4
essential
no
synonyms
lepA, yqxB, yqeQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0481 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,630,910  2,632,748
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The protein
Catalyzed reaction/ biological activity
GTP + H2O --> GDP + H+ + phosphate (according to UniProt)
Protein family
[wiki|TRAFAC class translation factor GTPase superfamily] (according to UniProt)
[wiki|Classic translation factor GTPase family] (according to UniProt)
[wiki|Domains]
[wiki|tr-type G domain] (aa 12-194) (according to UniProt)
Structure
[PDB|4QJT] (LepA bound to the ''Thermus thermophilus'' ribosome, 62% identity) [Pubmed|25104389]
[AF|P37949]
[wiki|Localization]
cytoplasm (Homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|3744083E3BB30A64D15360A12636665F76C40327|lepA]-[gene|757C51296E198849EAC1FFA4D37121D9085930A8|hemN]
description
[Pubmed|8757728]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9371469], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
There is a terminator between '[protein|search|lepA]' and '[protein|search|hemN]' with only little readthrough
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[gene|3744083E3BB30A64D15360A12636665F76C40327|lepA]→[gene|757C51296E198849EAC1FFA4D37121D9085930A8|hemN]

2024-04-06 21:37:18

ghost

65

39ed826203c90d2226c3efa52b0486b18d1b1c11

35FE32B4C189AAA5AF31CBF63C3202958F6B1550

Biological materials
Mutant
BKE25510 ([gene|3744083E3BB30A64D15360A12636665F76C40327|lepA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAATCTATCACTCCTACT,  downstream forward: _UP4_TAGAAGCCGCCGCAGTCTTG
BKK25510 ([gene|3744083E3BB30A64D15360A12636665F76C40327|lepA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAATCTATCACTCCTACT,  downstream forward: _UP4_TAGAAGCCGCCGCAGTCTTG
References
36815589,8757728,9371469,16479537,25378333,23662805,21300907,25491353,17307049,25104389,17110332,18362332

3744083E3BB30A64D15360A12636665F76C40327

Page visits: 5356

Time of last update: 2024-04-16 08:58:26

Author of last update: Jstuelk