lcfB

lcfB
168

long-chain fatty-acid-CoA ligase, involved in surfactin production

locus
BSU_10270
Molecular weight
56.41 kDa
pI
5.3
Protein length
Gene length
function
fatty acid degradation
product
long-chain fatty-acid-CoA ligase
essential
no
ec
6.2.1.3
synonyms
lcfB, yhfL

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,100,980  1,102,521
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The protein
Catalyzed reaction/ biological activity
long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)
activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]
Protein family
[wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)
Structure
[PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 35% identity) [pubmed|22560731]
[AF|O07610]
Paralogous protein(s)
[protein|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT], [protein|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI], [protein|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]
Expression and Regulation
Operons
genes
[gene|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB]
description
[Pubmed|17189250]
regulation
induced in the presence of long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]) [Pubmed|17189250]
down-regulated in response to [protein|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS] overexpression [pubmed|37338392]
regulatory mechanism
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]: repression, [Pubmed|17189250], in [regulon|protein:8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]: sRNA, [pubmed|37338392], in [regulon|protein:6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17189250], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB]

2024-02-20 22:25:47

Jstuelk

41

86F07F1B6A32339653818814B91C36B025A3D317

657B5D1C8CA91660DFE7B8238DA030366BAA51BF

Biological materials
Mutant
MGNA-B277 (yhfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1276 NBRP B. subtilis, Japan]
available in [wiki|Mohamed Marahiel]'s lab [Pubmed|20797616]
BKE10270 ([gene|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCCACCCCTTAAGT,  downstream forward: _UP4_TAAAATGAAAAAGCGAAGCG
BKK10270 ([gene|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTCCCACCCCTTAAGT,  downstream forward: _UP4_TAAAATGAAAAAGCGAAGCG
References
17189250,17919287,20797616,22560731

41B879EF69E2D4F5C1896A4709567B88FD8AC5B7

Page visits: 3546

Time of last update: 2024-03-28 20:00:27

Author of last update: Melvin.boenninger