lcfA

lcfA
168

long chain acyl-CoA synthetase, involved in surfactin production

locus
BSU_28560
Molecular weight
62.52 kDa
pI
6.12
Protein length
Gene length
function
fatty acid degradation
product
long chain acyl-CoA synthetase
essential
no
ec
6.2.1.3
synonyms
lcfA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,918,646  2,920,328
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The protein
Catalyzed reaction/ biological activity
long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)
activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]
Protein family
[wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)
Structure
[PDB|3R44] (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 28% identity) [pubmed|22560731]
[AF|P94547]
Paralogous protein(s)
[protein|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB], [protein|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT], [protein|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]-[gene|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]-[gene|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|fadB]-[gene|251600E5FD52DC0352A123F1224C037577F5A09C|etfB]-[gene|ACA8FF923D72CC99F447E47F6A1381FEAD9DAB09|etfA]
description
[Pubmed|17189250]
regulation
induced by long chain acyl-CoA (C14 ... C20) ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]) [Pubmed|17189250]
regulatory mechanism
[protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR]: repression, [Pubmed|17189250], in [regulon|protein:8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|fadR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|21398533], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21398533], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]→[gene|ACA8FF923D72CC99F447E47F6A1381FEAD9DAB09|etfA]

2024-03-23 18:46:55

Jstuelk

62

d18d49394aac4cec0002a2695f8ea4b1c4cda4f5

DEE7D2778BB88C7028C79F5FEDED5CD757497033

Biological materials
Mutant
available in [wiki|Mohamed Marahiel]'s lab [Pubmed|20797616]
1A852 ( ''lcfA''::''cat''), [Pubmed|17085570], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A852&Search=1A852 BGSC]
BKE28560 ([gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA,  downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
BKK28560 ([gene|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA,  downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
References
17189250,17919287,20797616,17085570,22900538,22560731,31113899

7AC5217CA35F8E134ABB35D74F1BA5E684D2E058

Page visits: 5205

Time of last update: 2024-03-29 15:40:10

Author of last update: Melvin.boenninger