pxpC
168
subunit of ATP-dependent 5-oxoprolinase, antagonist of [protein|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|pxpB], control of the [wiki|phosphorelay]
locus
BSU_04090
Molecular weight
36.40 kDa
pI
5.47
function
detoxification of 5-oxoproline, control of the [wiki|phosphorelay], initiation of [wiki|sporulation]
product
subunit of 5-oxoprolinase, antagonist of [wiki|KipI]
essential
no
synonyms
pxpC, kipA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1984 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
460,592 461,599
Phenotypes of a mutant
no growth with 5-oxoproline as single source of nitrogen [pubmed|28830929]
reduced growth with ammonium as source of nitrogen due to the accumulation of toxic 5-oxoproline [pubmed|28830929]
The protein
Catalyzed reaction/ biological activity
5-oxoproline + ATP --> glutamate + ADP + Pi [pubmed|28830929]
Protein family
PxpC family (single member, according to UniProt)
Structure
[PDB|5DUD] ( the YbgJ-YbgK complex from E. coli, 36% identity)
[AF|Q7WY77]
Kinetic information
Km (5-oxoproline): 39 M [pubmed|28830929]
Expression and Regulation
Operons
genes
[gene|77F758CE9A59AC41F283CB1F5099097386133799|pxpA]-[gene|9AD419E3CB86C54A0F11E96C82E80A357C6B23D2|ycsG]-[gene|312E2A4ADFBCB9DD9AD9EEEC9FE53F3932AF5DBA|ycsI]-[gene|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|pxpB]-[gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]-[gene|7DA9A79876C546B78B716A64706A3A3716018C2E|kipR]-[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]
description
[Pubmed|9334321]
regulation
induced in the presence of 5-oxoproline [pubmed|28830929]
regulatory mechanism
[protein|7DA9A79876C546B78B716A64706A3A3716018C2E|kipR]: repression, [Pubmed|9334321], in [regulon|protein:7DA9A79876C546B78B716A64706A3A3716018C2E|kipR regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|9334321], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
Biological materials
Mutant
BKE04090 ([gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGGGCTTTAATACTTTCA, downstream forward: _UP4_CTGACATAGCAAAGGGGATG
BKK04090 ([gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGGGCTTTAATACTTTCA, downstream forward: _UP4_CTGACATAGCAAAGGGGATG
References
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Time of last update: 2024-04-18 20:45:27
Author of last update: Melvin.boenninger