ispE
168
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_00460
Molecular weight
31.57 kDa
pI
6.35
function
MEP pathway of isoprenoid biosynthesis
product
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
essential
yes
ec
2.7.1.148
synonyms
ispE, yabH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1947 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
53,516 54,385
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (according to UniProt)
4-CDP-2-C-methyl-D-erythritol + ATP --> 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H+ (according to UniProt)
Protein family
GHMP kinase family (together with [protein|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|galK] and [protein|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]) (according to UniProt)
Structure
[PDB|1UEK] (from ''E. coli'', 38% identity) [Pubmed|12771135]
[AF|P37550]
Expression and Regulation
Operons
genes
[gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
description
[Pubmed|22383849]
regulation
negative autoregulation [Pubmed|7638212]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, [Pubmed|7638212], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
Biological materials
Mutant
MGNA-B910 (yabH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1909 NBRP B. subtilis, Japan]
References
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Time of last update: 2024-04-18 14:53:22
Author of last update: Jstuelk