hrcA
168
transcriptional repressor of class I heat-shock genes
locus
BSU_25490
Molecular weight
38.87 kDa
pI
6.08
function
regulation of chaperone gene expression
product
transcriptional repressor
essential
no
synonyms
hrcA, yqxE, yqeS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1420 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,628,606 2,629,637
Phenotypes of a mutant
reduced survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]
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The protein
Catalyzed reaction/ biological activity
transcription repressor of the ''[gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]'' and ''[gene|0D8B3F1F86FDBAE0F3B3651F456340F147B394AF|groES]-[gene|8F3DEE8828CA6BAAAF6DB2DA4349EF7AA9DF89F8|groEL]'' operons (at low/ normal temperatures)
Protein family
hrcA family (single member, according to UniProt)
[wiki|Cofactors]
[protein|8F3DEE8828CA6BAAAF6DB2DA4349EF7AA9DF89F8|groEL] acts as co-repressor
Structure
[PDB|1STZ] (from Thermotoga maritima, 27% identity) [pubmed|15979091]
[AF|P25499]
Modification
phosphorylated on arginine residues [Pubmed|24263382]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
description
[Pubmed|9023197]
regulation
induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]
regulatory mechanism
[protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]: repression, [Pubmed|1339421], in [regulon|protein:2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1339421], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE25490 ([gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATCATCACCTCTGTTA, downstream forward: _UP4_TAAGGGAATTTTGGCAAATT
BKK25490 ([gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATCATCACCTCTGTTA, downstream forward: _UP4_TAAGGGAATTTTGGCAAATT
labs
[wiki|Wolfgang Schumann], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]
[wiki|Thomas Wiegert], University of Bayreuth, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/Thomas Wiegert-Dateien/Thomas Wiegert.html Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-04-18 05:40:03
Author of last update: Jstuelk