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Categories containing this gene/protein


utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons


AbrB regulon, CcpA regulon, GntR regulon

The GntR regulon


Gene


  • Coordinates on the chromosome (coding sequence): 4,113,417 -> 4,114,148
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

  • GntR family of transcription factors
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

  • gluconate acts as molecular inducer
  • Structure

    Localization

    Interactions

    Additional information

    Expression and Regulation


    Operon

  • gntR-gntK-gntP-gntZ PubMed1PubMed2
  • Sigma factor

  • SigA PubMed
  • Regulation

  • induced by gluconate (GntR) PubMed
  • repressed by glucose (CcpA) PubMed
  • Regulatory mechanism

  • GntR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed
  • Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    1. Error occurred: cannot get document summary PMID: 220817675 Majidian P, Kuse J, Tanaka K, Najafi H, Zeinalabedini M, Takenaka S, Yoshida KI

    Bacillus subtilis GntR regulation modified to devise artificial transient induction systems

    J Gen Appl Microbiol. 2017 Jan 25;62(6):277-285. doi: 10.2323/jgam.2016.05.004. Epub 2016 Nov 8. PubMed PMID: 27829583.
    Marciniak BC, Pabijaniak M, de Jong A, Dűhring R, Seidel G, Hillen W, Kuipers OP

    High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

    BMC Genomics. 2012 Aug 17;13:401. doi: 10.1186/1471-2164-13-401. PubMed PMID: 22900538; PubMed Central PMCID: PMC3463425.
    Schumacher MA, Sprehe M, Bartholomae M, Hillen W, Brennan RG

    Structures of carbon catabolite protein A-(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators

    Nucleic Acids Res. 2011 Apr;39(7):2931-42. doi: 10.1093/nar/gkq1177. Epub 2010 Nov 23. PubMed PMID: 21106498; PubMed Central PMCID: PMC3074128.
    Yoshida K, Ishio I, Nagakawa E, Yamamoto Y, Yamamoto M, Fujita Y

    Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome

    Microbiology. 2000 Mar;146 ( Pt 3):573-9. PubMed PMID: 10746760.
    Miwa Y, Nagura K, Eguchi S, Fukuda H, Deutscher J, Fujita Y

    Catabolite repression of the Bacillus subtilis gnt operon exerted by two catabolite-responsive elements

    Mol Microbiol. 1997 Mar;23(6):1203-13. PubMed PMID: 9106211.
    Reizer J, Bergstedt U, Galinier A, Küster E, Saier MH Jr, Hillen W, Steinmetz M, Deutscher J

    Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr

    J Bacteriol. 1996 Sep;178(18):5480-6. PubMed PMID: 8808939; PubMed Central PMCID: PMC178371.
    Fujita Y, Miwa Y, Galinier A, Deutscher J

    Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr

    Mol Microbiol. 1995 Sep;17(5):953-60. PubMed PMID: 8596444.
    Yoshida K, Fujita Y, Sarai A

    Missense mutations in the Bacillus subtilis gnt repressor that diminish operator binding ability

    J Mol Biol. 1993 May 20;231(2):167-74. PubMed PMID: 8510140.
    Miwa Y, Fujita Y

    Promoter-independent catabolite repression of the Bacillus subtilis gnt operon

    J Biochem. 1993 Jun;113(6):665-71. PubMed PMID: 8370661.
    Fujita Y, Miwa Y

    Catabolite repression of the Bacillus subtilis gnt operon mediated by the CcpA protein

    J Bacteriol. 1994 Jan;176(2):511-3. PubMed PMID: 8288545; PubMed Central PMCID: PMC205075.
    Yoshida K, Ohmori H, Miwa Y, Fujita Y

    Bacillus subtilis gnt repressor mutants that diminish gluconate-binding ability

    J Bacteriol. 1995 Aug;177(16):4813-6. PubMed PMID: 7642511; PubMed Central PMCID: PMC177250.
    Yoshida K, Miwa Y, Ohmori H, Fujita Y

    Analysis of an insertional operator mutation (gntOi) that affects the expression level of the Bacillus subtilis gnt operon, and characterization of gntOi suppressor mutations

    Mol Gen Genet. 1995 Sep 20;248(5):583-91. PubMed PMID: 7476858.
    Yoshida K, Miwa Y, Ohmori H, Fujita Y

    Analysis of an insertional operator mutation (gntOi) that affects the expression level of the Bacillus subtilis gnt operon, and characterization of gntOi suppressor mutations

    Mol Gen Genet. 1995 Sep 20;248(5):583-91. PubMed PMID: 7476858.
    Fujita Y, Fujita T

    The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator

    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4524-8. PubMed PMID: 3037520; PubMed Central PMCID: PMC305122.
    Fujita Y, Fujita T, Miwa Y, Nihashi J, Aratani Y

    Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis

    J Biol Chem. 1986 Oct 15;261(29):13744-53. PubMed PMID: 3020045.
    Fujita Y, Fujita T, Miwa Y, Nihashi J, Aratani Y

    Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis

    J Biol Chem. 1986 Oct 15;261(29):13744-53. PubMed PMID: 3020045.
    Fujita Y, Nihashi J, Fujita T

    The characterization and cloning of a gluconate (gnt) operon of Bacillus subtilis

    J Gen Microbiol. 1986 Jan;132(1):161-9. PubMed PMID: 3011959.
    Miwa Y, Fujita Y

    Purification and characterization of a repressor for the Bacillus subtilis gnt operon

    J Biol Chem. 1988 Sep 15;263(26):13252-7. PubMed PMID: 2843515.
    Fujita Y, Fujita T

    Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon

    Nucleic Acids Res. 1986 Feb 11;14(3):1237-52. PubMed PMID: 2419835; PubMed Central PMCID: PMC339500.
    Fujita Y, Fujita T, Miwa Y

    Evidence for posttranscriptional regulation of synthesis of the Bacillus subtilis Gnt repressor

    FEBS Lett. 1990 Jul 2;267(1):71-4. PubMed PMID: 2163901.
    Miwa Y, Fujita Y

    Determination of the cis sequence involved in catabolite repression of the Bacillus subtilis gnt operon; implication of a consensus sequence in catabolite repression in the genus Bacillus

    Nucleic Acids Res. 1990 Dec 11;18(23):7049-53. PubMed PMID: 2124676; PubMed Central PMCID: PMC332768.
    Reizer A, Deutscher J, Saier MH Jr, Reizer J

    Analysis of the gluconate (gnt) operon of Bacillus subtilis

    Mol Microbiol. 1991 May;5(5):1081-9. PubMed PMID: 1659648.
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