glyA
168
serine hydroxymethyltransferase
locus
BSU_36900
Molecular weight
45.33 kDa
pI
5.47
function
biosynthesis of glycine
product
serine hydroxymethyltransferase
essential
no
ec
2.1.2.1
synonyms
glyA, glyC, ipc-34d
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0112 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,789,190 3,790,437
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
inactivation of ''[gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]'' suppresses the synthetic lethality of the ''[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA] [gene|53B760316B19E142FC3ECAD2CFAA540377B08BBC|defB]'' double mutant [Pubmed|27983482]
poor growth [pubmed|28189581]
poorly transformable [pubmed|28189581]
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The protein
Catalyzed reaction/ biological activity
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O --> (6S)-5,6,7,8-tetrahydrofolate + L-serine (according to UniProt)
generation of 5-formyl-THF as a side product
Protein family
SHMT family (single member, according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|2VGU] (complex with L-serine, Geobacillus stearothermophilus), [PDB|2VI8] (Geobacillus stearothermophilus)
[AF|P39148]
Modification
phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]
description
[Pubmed|22383849]
regulation
induced by glycine limitation ([regulon|T-box|]) [Pubmed|19258532]
regulatory mechanism
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
T-box: RNA switch, in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11591660], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE36900 (''[gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]''::''ermC'') (available in the BGSC and in [wiki|Jrg Stlke]'s lab) [pubmed|28189581]
BKE36900 ([gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGCGAGATCCTCTCCT, downstream forward: _UP4_TAAGATCCTAAAACCCGCTT
BKK36900 ([gene|BAE8062DA8538C9C83FFBA9684BECE9613EEFD2F|glyA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36900 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGCGAGATCCTCTCCT, downstream forward: _UP4_TAAGATCCTAAAACCCGCTT
References
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Time of last update: 2024-04-16 19:08:01
Author of last update: Jstuelk