dinG
168
ATP-dependent DNA helicase
locus
BSU_22400
Molecular weight
105.83 kDa
pI
5.82
function
response to DNA damage
product
ATP-dependent DNA helicase
essential
no
synonyms
dinG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2176 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,349,671 2,352,466
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The protein
Catalyzed reaction/ biological activity
binds to abasic and RNA base DNA lesions [pubmed|37250769]
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|Domains]
[wiki|Exonuclease domain] (aa 7-162) (according to UniProt)
[wiki|Helicase ATP-binding domain] (aa 250-510) (according to UniProt)
[wiki|Helicase C-terminal domain] (aa 741-897) (according to UniProt)
Structure
[PDB|4A15] (from Thermoplasma acidophilum, corresponds to aa 257 ... 913 of DinG, 22% identity) [pubmed|22081108]
[AF|P54394]
Paralogous protein(s)
[protein|0F9B77CE6C662D2F249C7E33E5C553A74C390259|ypvA]
[wiki|Localization]
Cytoplasm (Homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|A0001681244113B808757281C1B70C0C94F28A27|panB]-[gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]-[gene|EE712C1EC9E9C371847C83F7D75C189B276CD4B6|panD]-[gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]-[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A423 (dinG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/423 NBRP B. subtilis, Japan]
BKE22400 ([gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATGGACACCTCAA, downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC
BKK22400 ([gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22400 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATGGACACCTCAA, downstream forward: _UP4_TAAAAGCCGCATAAAGCGGC
References
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Time of last update: 2024-04-19 12:53:46
Author of last update: Jstuelk