des

des
168

phospholipid desaturase

locus
BSU_19180
Molecular weight
40.55 kDa
pI
9.88
Protein length
Gene length
function
adaptation of membrane fluidity at low temperatures
product
phospholipid desaturase
essential
no
synonyms
des, yocE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3239 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,089,396  2,090,454
Phenotypes of a mutant
cold-sensitive [Pubmed|19820084]
a strain lacking the [gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB] operon and the [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des] gene exhibits strongly reduced membrane fluidity and eventual growth arrest [pubmed|35037270]
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The protein
Catalyzed reaction/ biological activity
introduces double bonds into long chain fatty acids [Pubmed|21665975]
Protein family
Fatty acid desaturase type 1 family (single member, according to UniProt)
[wiki|Cofactors]
[protein|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des] receives electrons from ferredoxin ([protein|4164F07C581320D67FDC1ED5014272B81702A371|fer]) or from either of the two flavodoxins, [protein|0932DDC285635C02067A3302CEDA302FE4269179|ykuN] or [protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|ykuP], the three proteins can functionally replace each other [Pubmed|21665975]
Structure
[AF|O34653]
[wiki|Localization]
integral membrane protein [Pubmed|21665975]
Expression and Regulation
Operons
genes
[gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
description
[Pubmed|10559169]
regulation
induced upon cold shock ([protein|search|DesR]) [Pubmed|11285232]
regulatory mechanism
[protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR]: activation, [Pubmed|11285232], [Pubmed|19820084], in [regulon|protein:F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|desR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10559169], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
in strains evolved for growth at low pressure, 'des' expression is significantly increased as compared to the wild type [PubMed|22416764]
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[gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]

2024-03-14 21:09:10

ghost

56

35ec1b5fa68baac6e2e8746b4d49e94f1548d815

E5995014EB7214334800062D275177EDB8211CBE

Biological materials
Mutant
MGNA-A319 (yocE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/319 NBRP B. subtilis, Japan]
1A835 ( ''des''::''kan''), [Pubmed|11285232], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A835&Search=1A835 BGSC]
BKE19180 ([gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTAAGTATCCTCTCAT,  downstream forward: _UP4_TGATGATAAAGGAGGTCTTC
BKK19180 ([gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19180 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTAAGTATCCTCTCAT,  downstream forward: _UP4_TGATGATAAAGGAGGTCTTC
labs
[wiki|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]
References
Reviews
19363032
Original Publications
19164152,11251847,12399512,9555904,10559169,11285232,12207704,12730185,19820084,12207704,20581210,22416764,18154726,21665975,26647299,28461449,30087203,35037270,36815589

644C0C354B72FC07222EE45F4D2E0E57434B5EB5

Page visits: 3344

Time of last update: 2024-03-29 00:23:07

Author of last update: Jstuelk