dctP
168
uptake of succinate, fumarate, malate and oxaloacetate via proton symport
locus
BSU_04470
Molecular weight
45.28 kDa
pI
8.67
function
uptake of succinate, fumurate, malate and oxaloacetate
product
C4-dicarboxylate transport protein
essential
no
synonyms
dctP, ydbH, dctA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1301 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
500,166 501,431
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The protein
Catalyzed reaction/ biological activity
uptake of succinate, fumurate, malate and oxaloacetate via proton symport [Pubmed|20363944]
acts as co-sensor for [protein|9A96572B101358768EB20EE9438BE0E1424A4130|dctS] [Pubmed|24375102]
Protein family
[wiki|dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family] (according to UniProt)
Structure
[PDB|4KY0] (the glutamate transporter of ''Thermococcus kodakarensis'', 31% identity, 71% similarity) [Pubmed|24013209]
[AF|P96603]
Paralogous protein(s)
[protein|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT], [protein|622856EE642C42247C658DBD57E6A18C6E81FE58|gltP]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]
description
[Pubmed|10708364]
regulation
part of the iron sparing response, strong down-regulation in a'' [protein|search|fur]'' mutant ([protein|search|Fur], [protein|search|FsrA]) [Pubmed|22389480]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: indirect effect, in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10708364], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|9A96572B101358768EB20EE9438BE0E1424A4130|dctS]-[gene|97C34F6FA0B89D93369150E9A8AB3579332B07BC|dctR]-[gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]
description
[Pubmed|10708364]
regulation
part of the iron sparing response, strong down-regulation in a'' [protein|search|fur]'' mutant ([protein|search|Fur], [protein|search|FsrA]) [Pubmed|22389480]
Other regulations
[protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]: translation inhibition, [Pubmed|22389480]
Biological materials
Mutant
BKE04470 ([gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCTTCATCCCCCTGT, downstream forward: _UP4_TGAAAAAAGAACGGCCCATC
BKK04470 ([gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCTTCATCCCCCTGT, downstream forward: _UP4_TGAAAAAAGAACGGCCCATC
GP2600 ([gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]::tet comIQ12L) (in DK1042) available in [wiki|Jörg Stülke]'s lab
GP4218 ([gene|107DDCC7B6AA2D7CB02E53F043A93CF05C679081|dctP]::tet), available in Jörg Stülke's lab
References
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Time of last update: 2024-04-19 18:19:47
Author of last update: Christoph.elfmann