cggR

cggR
168

repressor of the glycolytic [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] operon, [wiki|DeoR family]

locus
BSU_33950
Molecular weight
37.23 kDa
pI
5.68
Protein length
Gene length
function
transcriptional regulator
product
central glycolytic genes regulator
essential
no
synonyms
cggR, yvbQ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2390 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,482,752  3,483,774
Phenotypes of a mutant
suppresses the growth defect of [gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR] mutants on gluconeogenic substrates [Pubmed|16272399]
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The protein
Catalyzed reaction/ biological activity
transcription repression of the glycolytic ''[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]'' operon
Protein family
SorC transcriptional regulatory family (with [protein|E9C4C569F18B3335DA9C7FB5B17C87D661E62669|deoR], according to UniProt)
[wiki|Domains]
DNA binding domain (H-T-H motif) (3756)
Structure
[PDB|3BXH] (in complex with fructose-6-phosphate) [pubmed|18554327]
[PDB|2OKG] (effector binding domain) [pubmed|18554327]
[PDB|7OYK] (complex of the DNA-binding domain with the operator DNA) [pubmed|34726169]
[AF|O32253]
Effectors of protein activity
fructose 1.6-bisphosphate [Pubmed|29923648,12622823] and dihydroxyacetone phosphate, glucose-6-phosphate and fructose-6-phosphate [Pubmed|18554327] act as inducer and result in release of [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] from the DNA
[wiki|Localization]
cytoplasma [Pubmed|20572937]
Expression and Regulation
Operons
genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
regulation
expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] [Pubmed|11489127]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [Pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]→[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]

2024-03-05 10:12:07

Jstuelk

106

7b5a04e0f51be88bb9a430514dd0cb9d39ea3a2c

24906FF9311EDEA4AFD94747BD67B2022E642FAB

genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]
description
[Pubmed|11489127]
regulation
expression induced by glucose (10 fold) ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [Pubmed|12850135,12622823]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]→[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]

2024-03-10 07:41:01

Jstuelk

86

42c171759b28a8125169e493f230a3f3dfc6e9b8

312D000A710534F6E0BADE1077DFF609B6028623

additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-A463 (yvbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/463 NBRP B. subtilis, Japan]
GP311 (in frame deletion), available in [wiki|Jörg Stülke]'s lab
SM-NB7 (cggR-spc), available in [wiki|Anne Galinier]'s and [wiki|Boris Görke]'s labs
BKE33950 ([gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33950 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGACTCAAACGTTCCTTC,  downstream forward: _UP4_TAATCCCTCAATATAAATAT
BKK33950 ([gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33950 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGACTCAAACGTTCCTTC,  downstream forward: _UP4_TAATCCCTCAATATAAATAT
Expression vectors
pGP705 (N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP504 (in [wiki|pAC7]), pGP509 (in [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab
Antibody
available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
Reviews
20408793
Original Publications
20462860,22190493,23280112,21815947,19193632,12850135,12622823,18186488,10799476,11489127,12123463,12634343,18052209,17293407,20361740,20444087,18554327,25099370,26883633,16272399,29923648,30082753,34726169

ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160

Page visits: 6552

Time of last update: 2024-03-28 14:01:02

Author of last update: Jstuelk