birA

birA
168

trigger enzyme

locus
BSU_22440
Molecular weight
36.03 kDa
pI
6.77
Protein length
Gene length
function
regulation of biotin synthesis, addition of biotin to proteins
product
trigger enzyme
essential
yes
ec
6.3.4.15
synonyms
birA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1654 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,354,918 → 2,355,895
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
transcriptional repressor of the ''[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]'' operon [Pubmed|8763940,8892842] '''
ATP + biotin + L-lysyl-[protein] --> AMP + diphosphate + H+ + N6-biotinyl-L-lysyl-[protein] (according to UniProt)
Protein family
biotin--protein ligase family (single member, according to UniProt)
[wiki|Domains]
[wiki|BPL/LPL catalytic domain] (aa 74-262) (according to UniProt)
Structure
[PDB|4HA8]
[AF|P0CI75]
Effectors of protein activity
biotinoyl-5'-AMP acts as co-repressor for DNA binding [pubmed|28466579]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
description
[Pubmed|20308541]
regulation
induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]→[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]

2024-01-29 08:50:51

ghost

48

53bf67b167bd77fe013a5e22a2f6ddcbf0c547e2

73042872A92944881DD9D94DF293F1857E4735FB

Biological materials
Expression vectors
pGP2690: expression in ''E. coli'', with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]' lab
pGP2700: expression in ''B. subtilis'', with N-terminal Strep-tag, in [wiki|pGP380], available in [wiki|Jörg Stülke]' lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pBP629 (in [wiki|pAC7]), available in [wiki|Fabian Commichau]'s lab
Antibody
available in [wiki|Jörg Stülke]'s lab
References
Reviews
12368242,21437340,26350309,18086213
Original Publications
7730294,8763940,8892842,11717296,20308541,23894131,24189073,24816803,22437830,28466579

F761DBCE3429C99159F8F7CD8596FD9CF2D0589D

Page visits: 9172

Time of last update: 2024-03-28 10:47:51

Author of last update: Melvin.boenninger