bioI

bioI
168

cytochrome P450 enzyme

locus
BSU_30190
Molecular weight
44.70 kDa
pI
6.2
Protein length
395 aaSequenceBlast
Gene length
1188 bpSequenceBlast
function
biosynthesis of biotin
product
cytochrome P450 enzyme
essential
no
synonyms
bioI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,089,226  3,090,413
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The protein
Catalyzed reaction/ biological activity
formation of pimelic acid via a multiple-step oxidative cleavage of long-chain fatty acids
C2-C8-saturated long-chain fatty acyl-[ACP] + 3 O2 + 2 reduced [flavodoxin] --> fatty aldehyde + 3 H+ + 3 H2O + 2 oxidized [flavodoxin] + pimeloyl-[ACP] (according to UniProt)
Protein family
[wiki|cytochrome P450] family (according to UniProt)
Structure
[PDB|3EJB], [PDB|3EJE] (complex with [protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA]) [Pubmed|18838690]
[AF|P53554]
Paralogous protein(s)
[protein|A95C28DEE73AD25E28C45B88A7C03C92835F9705|cypA], [protein|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|yjiB], [protein|EF0F13BB682791E167EFC350BF029B08E8A1F410|pksS]
Expression and Regulation
Operons
genes
[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
description
[Pubmed|8763940,8892842]
regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, [Pubmed|8763940,8892842], in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]
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[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]→[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]

2024-04-18 06:53:51

ghost

44

8e74711c48d33750c45114afa6e28dd08cdf4ea5

F7255B320407AE19B7B38F8E863B5EF4A9BF3D52

Biological materials
Mutant
MGNA-A804 (bioI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/804 NBRP B. subtilis, Japan]
BKE30190 ([gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30190 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT,  downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG
BKK30190 ([gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30190 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTGATTTTCTCCTTTCT,  downstream forward: _UP4_TAAGCCTAAGAATGTGAGTG
References
Reviews
21437340
Original Publications
18838690,8763940,8892842,15449931,15449930,12538057,20658980,11368323,11472016,14737344,12943422,28196402,28898812

8FBC9A1AC108DC99E275D7A27C18C797D3CDB936

Page visits: 2879

Time of last update: 2024-04-19 11:27:12

Author of last update: Melvin.boenninger