SubtiBank SubtiBank

It will happen very soon:

International Conference on Bacilli and Gram-positive bacteria

June 11 - 15, 2017, Berlin, Germany

Check out the program here

Categories containing this gene/protein


utilization of specific carbon sources

This gene is a member of the following regulons


AbrB regulon, CcpA regulon

Gene


  • Coordinates on the chromosome (coding sequence): 327,618 -> 329,597
  • The protein


    Catalyzed reaction/ biological activity

  • Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides (according to Swiss-Prot)
  • Protein family

  • glycosyl hydrolase 13 family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 1BAG (complex with maltopentaose)
  • Localization

  • secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed
  • Interactions

    Additional information

    Expression and Regulation


    Operon

  • amyE PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • repressed by glucose (CcpA) PubMed
  • Regulatory mechanism

  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed
  • Additional information

    Biological materials


    Mutant

  • GP550 (cat), available in Stülke lab
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Gupta M, Rao KK

    Phosphorylation of DegU is essential for activation of amyE expression in Bacillus subtilis

    J Biosci. 2014 Dec;39(5):747-52. PubMed PMID: 25431404.
    Yang H, Liu L, Shin HD, Chen RR, Li J, Du G, Chen J

    Structure-based engineering of histidine residues in the catalytic domain of α-amylase from Bacillus subtilis for improved protein stability and catalytic efficiency under acidic conditions

    J Biotechnol. 2013 Mar 10;164(1):59-66. doi: 10.1016/j.jbiotec.2012.12.007. Epub 2012 Dec 20. PubMed PMID: 23262127.
    Marciniak BC, Pabijaniak M, de Jong A, Dűhring R, Seidel G, Hillen W, Kuipers OP

    High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

    BMC Genomics. 2012 Aug 17;13:401. doi: 10.1186/1471-2164-13-401. PubMed PMID: 22900538; PubMed Central PMCID: PMC3463425.
    Inaoka T, Ochi K

    Scandium stimulates the production of amylase and bacilysin in Bacillus subtilis

    Appl Environ Microbiol. 2011 Nov;77(22):8181-3. doi: 10.1128/AEM.06205-11. Epub 2011 Sep 23. PubMed PMID: 21948839; PubMed Central PMCID: PMC3209005.
    Zhao W, Zheng J, Zhou HB

    Hybrid on-line optimal control strategy for producing α-amylase by Bacillus subtilis

    Biosci Biotechnol Biochem. 2011;75(4):694-9. Epub 2011 Apr 22. PubMed PMID: 21512239.
    Chumsakul O, Takahashi H, Oshima T, Hishimoto T, Kanaya S, Ogasawara N, Ishikawa S

    Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation

    Nucleic Acids Res. 2011 Jan;39(2):414-28. doi: 10.1093/nar/gkq780. Epub 2010 Sep 3. PubMed PMID: 20817675; PubMed Central PMCID: PMC3025583.
    Voigt B, Antelmann H, Albrecht D, Ehrenreich A, Maurer KH, Evers S, Gottschalk G, van Dijl JM, Schweder T, Hecker M

    Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis

    J Mol Microbiol Biotechnol. 2009;16(1-2):53-68. doi: 10.1159/000142894. Epub 2008 Oct 29. PubMed PMID: 18957862.
    Nicholson WL, Park YK, Henkin TM, Won M, Weickert MJ, Gaskell JA, Chambliss GH

    Catabolite repression-resistant mutations of the Bacillus subtilis alpha-amylase promoter affect transcription levels and are in an operator-like sequence

    J Mol Biol. 1987 Dec 20;198(4):609-18. PubMed PMID: 3123701.
    Henkin TM, Grundy FJ, Nicholson WL, Chambliss GH

    Catabolite repression of alpha-amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacl and galR repressors

    Mol Microbiol. 1991 Mar;5(3):575-84. PubMed PMID: 1904524.
    No login