alkA
168
DNA-3-methyladenine glycosylase
locus
BSU_01800
Molecular weight
35.48 kDa
pI
8.15
function
adaptive response to alkylative DNA damage
product
DNA-3-methyladenine glycosylase
essential
no
ec
3.2.2.21
synonyms
alkA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0122 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
202,547 203,458
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The protein
Catalyzed reaction/ biological activity
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to UniProt)
Protein family
alkylbase DNA glycosidase AlkA family (with [protein|82CDC97D16426016CA7E75240C1A6D0D600F4375|yfjP], according to UniProt)
Structure
[PDB|2JHJ] (from Archaeoglobus fulgidus, 29% identity) [pubmed|17396151]
[AF|P37878]
Expression and Regulation
Operons
genes
[gene|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA]
description
[Pubmed|8376346]
regulatory mechanism
[protein|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]: activation, in [regulon|protein:0F8A564256A598DB3A36668FA0C984542FE1323F|adaA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8376346], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE01800 ([gene|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTCATTCTCCTTAT, downstream forward: _UP4_TGAATCATTTCAACTTTAAC
BKK01800 ([gene|297DF91EF3E91A6D2DC39BBC7FE8711AB7EBE509|alkA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTCATTCTCCTTAT, downstream forward: _UP4_TGAATCATTTCAACTTTAAC
References
Reviews
Original Publications
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Time of last update: 2024-04-19 01:18:50
Author of last update: Jstuelk