adaB

adaB
168

O6-methylguanine-DNA methyltransferase

locus
BSU_01820
Molecular weight
19.98 kDa
pI
6.15
Protein length
Gene length
function
adaptive response to alkylative DNA damage
product
O6-methylguanine-DNA methyltransferase
essential
no
ec
2.1.1.63
synonyms
adaB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0350 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
204,351 → 204,890
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The protein
Catalyzed reaction/ biological activity
6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] --> 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] --> thymidine in DNA + S-methyl-L-cysteinyl-[protein] (according to UniProt)
Protein family
MGMT family (with [protein|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt], according to UniProt)
Structure
[PDB|4BHB] (from Mycobacterium tuberculosis, 42% identity) [pubmed|23564173]
[AF|P19220]
Expression and Regulation
Operons
genes
[gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]-[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]
description
[Pubmed|2120677]
regulation
positive control by [protein|search|AdaA] [Pubmed|2120677]
regulatory mechanism
[protein|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]: activation, in [regulon|protein:0F8A564256A598DB3A36668FA0C984542FE1323F|adaA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2120677], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|0F8A564256A598DB3A36668FA0C984542FE1323F|adaA]→[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]

2024-03-14 12:06:58

ghost

72

216a299d21884eb43084923c354bd4664445d33d

BD6B9F925AA4A09B6EDF781E92DBC11359A0B270

Biological materials
Mutant
BKE01820 (Δ[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01820 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAGTAAAGACCAATAAAGGG,  downstream forward: _UP4_TGAAAGTGAATCATCTATAA
BKK01820 (Δ[gene|D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1|adaB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01820 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAGTAAAGACCAATAAAGGG,  downstream forward: _UP4_TGAAAGTGAATCATCTATAA
References
Reviews
22933559,24810496
Original Publications
2120677,23564173

D7E4E4A95CFDB3CFCC7432B08653644AF6876CA1

Page visits: 1986

Time of last update: 2024-03-27 22:12:22

Author of last update: Melvin.boenninger