SubtiBank SubtiBank

Categories containing this gene/protein


ATP synthesis, carbon core metabolism, most abundant proteins

This gene is a member of the following regulons


CcpA regulon, CodY regulon

Gene


  • Coordinates on the chromosome (coding sequence): 3,015,111 -> 3,016,298
  • The protein


    Catalyzed reaction/ biological activity

  • ATP + acetate = ADP + acetyl phosphate (according to Swiss-Prot)
  • Protein family

  • acetokinase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 2IIR (from Thermotoga maritima, 54% identity, 73% similarity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

    Additional information

    Expression and Regulation


    Operon

  • ackA PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • expression activated by glucose (2.5 fold) (CcpA) PubMed
  • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism

  • CodY: transcription activation PubMed
  • CcpA: transcription activation PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Biological materials


    Mutant

  • GP1902 (ackA::aphA3) (available in Jörg Stülke's lab)
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

    Antibody

    Labs working on this gene/protein


  • Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
  • References


    Reviews

    Ingram-Smith C, Martin SR, Smith KS

    Acetate kinase: not just a bacterial enzyme

    Trends Microbiol. 2006 Jun;14(6):249-53. Epub 2006 May 4. Review. PubMed PMID: 16678422.
    Wolfe AJ

    The acetate switch

    Microbiol Mol Biol Rev. 2005 Mar;69(1):12-50. Review. PubMed PMID: 15755952; PubMed Central PMCID: PMC1082793.

    Original publications

    Kosono S, Tamura M, Suzuki S, Kawamura Y, Yoshida A, Nishiyama M, Yoshida M

    Changes in the Acetylome and Succinylome of Bacillus subtilis in Response to Carbon Source

    PLoS One. 2015 Jun 22;10(6):e0131169. doi: 10.1371/journal.pone.0131169. eCollection 2015 Jun 22. PubMed PMID: 26098117; PubMed Central PMCID: PMC4476798.
    Schumacher MA, Sprehe M, Bartholomae M, Hillen W, Brennan RG

    Structures of carbon catabolite protein A-(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators

    Nucleic Acids Res. 2011 Apr;39(7):2931-42. doi: 10.1093/nar/gkq1177. Epub 2010 Nov 23. PubMed PMID: 21106498; PubMed Central PMCID: PMC3074128.
    Shivers RP, Dineen SS, Sonenshein AL

    Positive regulation of Bacillus subtilis ackA by CodY and CcpA: establishing a potential hierarchy in carbon flow

    Mol Microbiol. 2006 Nov;62(3):811-22. Epub 2006 Sep 21. PubMed PMID: 16995897.
    Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M

    A comprehensive proteome map of growing Bacillus subtilis cells

    Proteomics. 2004 Oct;4(10):2849-76. PubMed PMID: 15378759.
    Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J

    Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways

    Metab Eng. 2003 Apr;5(2):133-49. PubMed PMID: 12850135.
    Moir-Blais TR, Grundy FJ, Henkin TM

    Transcriptional activation of the Bacillus subtilis ackA promoter requires sequences upstream of the CcpA binding site

    J Bacteriol. 2001 Apr;183(7):2389-93. PubMed PMID: 11244084; PubMed Central PMCID: PMC95151.
    Turinsky AJ, Grundy FJ, Kim JH, Chambliss GH, Henkin TM

    Transcriptional activation of the Bacillus subtilis ackA gene requires sequences upstream of the promoter

    J Bacteriol. 1998 Nov;180(22):5961-7. PubMed PMID: 9811655; PubMed Central PMCID: PMC107671.
    Turinsky AJ, Grundy FJ, Kim JH, Chambliss GH, Henkin TM

    Transcriptional activation of the Bacillus subtilis ackA gene requires sequences upstream of the promoter

    J Bacteriol. 1998 Nov;180(22):5961-7. PubMed PMID: 9811655; PubMed Central PMCID: PMC107671.
    Grundy FJ, Waters DA, Allen SH, Henkin TM

    Regulation of the Bacillus subtilis acetate kinase gene by CcpA

    J Bacteriol. 1993 Nov;175(22):7348-55. PubMed PMID: 8226682; PubMed Central PMCID: PMC206879.
    Grundy FJ, Waters DA, Allen SH, Henkin TM

    Regulation of the Bacillus subtilis acetate kinase gene by CcpA

    J Bacteriol. 1993 Nov;175(22):7348-55. PubMed PMID: 8226682; PubMed Central PMCID: PMC206879.
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