SubtiBank SubtiBank

Categories containing this gene/protein


transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons


AbrB regulon, ScoC regulon, Spo0A regulon

Gene


  • Coordinates on the chromosome (coding sequence): 2,552,446 -> 2,552,619
  • Phenotypes of a mutant

  • altered cell death pattern in colonies PubMed
  • The protein


    Catalyzed reaction/ biological activity

    Protein family

    Paralogous protein(s)

  • SlrA
  • Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 1B0N (complex SinR-SinI) PubMed
  • Localization

    Interactions

  • SinR-SinI PubMed, K(D) 1.8 nM PubMed
  • Additional information

    Expression and Regulation


    Operon

  • sinI-sinR PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • activated under conditions that trigger sporulation by (Spo0A) interaction PubMed,PubMed
  • repressed during exponential growth (ScoC) PubMed
  • repressed during logrithmic growth (AbrB) PubMed
  • repressed during vegetative growth (SinR) PubMed
  • Regulatory mechanism

  • Spo0A: transcription activation, in a portion of cells PubMed
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed
  • SinR: transcription repression PubMed
  • Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • Biological materials


    Mutant

  • GP959 (spc), available in Jörg Stülke's lab
  • DS91 (spc) NCIB3610 derivate, available in Jörg Stülke's lab
  • 1S98 ( sinI::kan), PubMed, available at BGSC
  • GP1663 (yqhG-sinI-sinR-tasA), available in Jörg Stülke's lab
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Piggot P(1).

    Epigenetic switching: bacteria hedge bets about staying or moving.

    Curr Biol. 2010 Jun 8;20(11):R480-2. doi: 10.1016/j.cub.2010.04.020.DOI: 10.1016/j.cub.2010.04.020 PMID: 20541494 [Indexed for MEDLINE]

    Modelling of the SinI/SinR switch

    Hallinan JS(1), Misirli G, Wipat A.

    Evolutionary computation for the design of a stochastic switch for synthetic genetic circuits.

    Conf Proc IEEE Eng Med Biol Soc. 2010;2010:768-74. doi: 10.1109/IEMBS.2010.5626353.

    Original publications

    Ogura M

    Post-transcriptionally generated cell heterogeneity regulates biofilm formation in Bacillus subtilis

    Genes Cells. 2016 Apr;21(4):335-49. doi: 10.1111/gtc.12343. Epub 2016 Jan 28. PubMed PMID: 26819068.
    Norman TM, Lord ND, Paulsson J, Losick R

    Memory and modularity in cell-fate decision making

    Nature. 2013 Nov 28;503(7477):481-6. doi: 10.1038/nature12804. Epub 2013 Nov 20. PubMed PMID: 24256735; PubMed Central PMCID: PMC4019345.
    Newman JA, Rodrigues C, Lewis RJ

    Molecular basis of the activity of SinR protein, the master regulator of biofilm formation in Bacillus subtilis

    J Biol Chem. 2013 Apr 12;288(15):10766-78. doi: 10.1074/jbc.M113.455592. Epub 2013 Feb 21. PubMed PMID: 23430750; PubMed Central PMCID: PMC3624457.
    Asally M, Kittisopikul M, Rué P, Du Y, Hu Z, Çağatay T, Robinson AB, Lu H, Garcia-Ojalvo J, Süel GM

    Localized cell death focuses mechanical forces during 3D patterning in a biofilm

    Proc Natl Acad Sci U S A. 2012 Nov 13;109(46):18891-6. doi: 10.1073/pnas.1212429109. Epub 2012 Sep 24. PubMed PMID: 23012477; PubMed Central PMCID: PMC3503208.
    Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J

    RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y

    Mol Microbiol. 2011 Sep;81(6):1459-73. doi: 10.1111/j.1365-2958.2011.07777.x. Epub 2011 Aug 4. PubMed PMID: 21815947.
    Chai Y, Norman T, Kolter R, Losick R

    Evidence that metabolism and chromosome copy number control mutually exclusive cell fates in Bacillus subtilis

    EMBO J. 2011 Apr 6;30(7):1402-13. doi: 10.1038/emboj.2011.36. Epub 2011 Feb 15. PubMed PMID: 21326214; PubMed Central PMCID: PMC3094124.
    Chai Y, Kolter R, Losick R

    Reversal of an epigenetic switch governing cell chaining in Bacillus subtilis by protein instability

    Mol Microbiol. 2010 Oct;78(1):218-29. doi: 10.1111/j.1365-2958.2010.07335.x. PubMed PMID: 20923420; PubMed Central PMCID: PMC2998389.
    Chai Y, Chu F, Kolter R, Losick R

    Bistability and biofilm formation in Bacillus subtilis

    Mol Microbiol. 2008 Jan;67(2):254-63. Epub 2007 Nov 28. PubMed PMID: 18047568; PubMed Central PMCID: PMC2430929.
    Kearns DB, Chu F, Branda SS, Kolter R, Losick R

    A master regulator for biofilm formation by Bacillus subtilis

    Mol Microbiol. 2005 Feb;55(3):739-49. PubMed PMID: 15661000.
    Sánchez A, Olmos J

    Bacillus subtilis transcriptional regulators interaction

    Biotechnol Lett. 2004 Mar;26(5):403-7. PubMed PMID: 15104138.
    Shafikhani SH, Mandic-Mulec I, Strauch MA, Smith I, Leighton T

    Postexponential regulation of sin operon expression in Bacillus subtilis

    J Bacteriol. 2002 Jan;184(2):564-71. PubMed PMID: 11751836; PubMed Central PMCID: PMC139554.
    Shafikhani SH, Mandic-Mulec I, Strauch MA, Smith I, Leighton T

    Postexponential regulation of sin operon expression in Bacillus subtilis

    J Bacteriol. 2002 Jan;184(2):564-71. PubMed PMID: 11751836; PubMed Central PMCID: PMC139554.
    Shafikhani SH, Mandic-Mulec I, Strauch MA, Smith I, Leighton T

    Postexponential regulation of sin operon expression in Bacillus subtilis

    J Bacteriol. 2002 Jan;184(2):564-71. PubMed PMID: 11751836; PubMed Central PMCID: PMC139554.
    Scott DJ, Leejeerajumnean S, Brannigan JA, Lewis RJ, Wilkinson AJ, Hoggett JG

    Quaternary re-arrangement analysed by spectral enhancement: the interaction of a sporulation repressor with its antagonist

    J Mol Biol. 1999 Nov 12;293(5):997-1004. PubMed PMID: 10547280.
    Lewis RJ, Brannigan JA, Offen WA, Smith I, Wilkinson AJ

    An evolutionary link between sporulation and prophage induction in the structure of a repressor:anti-repressor complex

    J Mol Biol. 1998 Nov 13;283(5):907-12. Review. PubMed PMID: 9799632.
    Bai U, Mandic-Mulec I, Smith I

    SinI modulates the activity of SinR, a developmental switch protein of Bacillus subtilis, by protein-protein interaction

    Genes Dev. 1993 Jan;7(1):139-48. PubMed PMID: 8422983.
    Strauch MA

    In vitro binding affinity of the Bacillus subtilis AbrB protein to six different DNA target regions

    J Bacteriol. 1995 Aug;177(15):4532-6. PubMed PMID: 7635837; PubMed Central PMCID: PMC177209.
    Gaur NK, Cabane K, Smith I

    Structure and expression of the Bacillus subtilis sin operon

    J Bacteriol. 1988 Mar;170(3):1046-53. PubMed PMID: 3125149; PubMed Central PMCID: PMC210872.
    Kallio PT, Fagelson JE, Hoch JA, Strauch MA

    The transition state regulator Hpr of Bacillus subtilis is a DNA-binding protein

    J Biol Chem. 1991 Jul 15;266(20):13411-7. PubMed PMID: 1906467.
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