SubtiBank SubtiBank

Categories containing this gene/protein


utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

This gene is a member of the following regulons


DnaA regulon

The SacT regulon


Gene


  • Coordinates on the chromosome (coding sequence): 3,906,142 -> 3,906,972
  • The protein


    Catalyzed reaction/ biological activity

  • binding to the mRNA of the sacP-sacA operon, causes transcription antitermination (in presence of sucrose and absence of glucose)
  • Protein family

  • [PRD-containing transcription factors] bglG family (according to Swiss-Prot) BglG family of antiterminators
  • Paralogous protein(s)

  • LicT, SacY, GlcT
  • Kinetic information

    Domains

  • N-terminal RNA binding domain PubMed
  • 2 x PRD (PTS regulation domains) PubMed
  • Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

    Interactions

  • PtsH-SacT
  • Additional information

    Expression and Regulation


    Operon

  • ywcI-sacT PubMed
  • sacT PubMed
  • Sigma factor

    Regulation

  • repressed by casamino acids PubMed
  • Regulatory mechanism

  • DnaA: transcription repression PubMed
  • Additional information

    Biological materials


    Mutant

  • GP429 (spc), available in Jörg Stülke's lab
  • Expression vector

  • for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP166, available in Jörg Stülke's lab
  • for expression, purification of PRD-1 in E. coli with N-terminal His-tag, in pWH844: pGP426, available in Jörg Stülke's lab
  • for expression, purification of PRD-2 in E. coli with N-terminal His-tag, in pWH844: pGP427, available in Jörg Stülke's lab
  • for expression, purification of PRD-1 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP439, available in Jörg Stülke's lab
  • for expression, purification of PRD-2 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP440, available in Jörg Stülke's lab
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP571, available in Jörg Stülke's lab
  • for expression of the RNA-binding domain in B. subtilis, in pBQ200: pGP446, available in Jörg Stülke's lab
  • for expression, purification of sacT-full length in B. subtilis with C-terminal Strep-tag, in pGP382: pGP1064, available in Jörg Stülke's lab
  • for expression, purification of sacT-full length in B. subtilis with N-terminal Strep-tag, in pGP380: pGP1068, available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

  • GP1227 (spc, based on pGP1870), available in Jörg Stülke's lab
  • two-hybrid system

    FLAG-tag construct

  • GP1223 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein


    References


    Reviews

    Commichau FM, Stülke J

    Trigger enzymes: bifunctional proteins active in metabolism and in controlling gene expression

    Mol Microbiol. 2008 Feb;67(4):692-702. Epub 2007 Dec 11. Review. PubMed PMID: 18086213.
    Stülke J, Arnaud M, Rapoport G, Martin-Verstraete I

    PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria

    Mol Microbiol. 1998 Jun;28(5):865-74. Review. PubMed PMID: 9663674.

    Original publications

    Smith JL, Grossman AD

    In Vitro Whole Genome DNA Binding Analysis of the Bacterial Replication Initiator and Transcription Factor DnaA

    PLoS Genet. 2015 May 28;11(5):e1005258. doi: 10.1371/journal.pgen.1005258. eCollection 2015 May 28. PubMed PMID: 26020636; PubMed Central PMCID: PMC4447404.
    Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P

    Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis

    Science. 2012 Mar 2;335(6072):1103-6. doi: 10.1126/science.1206848. PubMed PMID: 22383849.
    Hübner S, Declerck N, Diethmaier C, Le Coq D, Aymerich S, Stülke J

    Prevention of cross-talk in conserved regulatory systems: identification of specificity determinants in RNA-binding anti-termination proteins of the BglG family

    Nucleic Acids Res. 2011 May;39(10):4360-72. doi: 10.1093/nar/gkr021. Epub 2011 Jan 28. PubMed PMID: 21278164; PubMed Central PMCID: PMC3105407.
    Mäder U, Homuth G, Scharf C, Büttner K, Bode R, Hecker M

    Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability

    J Bacteriol. 2002 Aug;184(15):4288-95. PubMed PMID: 12107147; PubMed Central PMCID: PMC135197.
    Arnaud M, Débarbouillé M, Rapoport G, Saier MH Jr, Reizer J

    In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis

    J Biol Chem. 1996 Aug 2;271(31):18966-72. PubMed PMID: 8702561.
    Debarbouille M, Arnaud M, Fouet A, Klier A, Rapoport G

    The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators

    J Bacteriol. 1990 Jul;172(7):3966-73. PubMed PMID: 2163394; PubMed Central PMCID: PMC213381.
    Arnaud M, Vary P, Zagorec M, Klier A, Debarbouille M, Postma P, Rapoport G

    Regulation of the sacPA operon of Bacillus subtilis: identification of phosphotransferase system components involved in SacT activity

    J Bacteriol. 1992 May;174(10):3161-70. PubMed PMID: 1577686; PubMed Central PMCID: PMC205982.
    Aymerich S, Steinmetz M

    Specificity determinants and structural features in the RNA target of the bacterial antiterminator proteins of the BglG/SacY family

    Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10410-4. PubMed PMID: 1279678; PubMed Central PMCID: PMC50348.
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