SubtiBank SubtiBank
'

Categories containing this gene/protein


protein modification, germination, membrane proteins, phosphoproteins

This gene is a member of the following regulons


Gene


  • Coordinates on the chromosome (coding sequence): 1,651,142 -> 1,653,088
  • Phenotypes of a mutant

  • unable to germinate in response to muropeptides PubMed
  • reduced growth at high salt PubMed
  • The protein


    Catalyzed reaction/ biological activity

  • ATP a protein = ADP a phosphoprotein (according to Swiss-Prot)
  • phosphorylation of WalR on Thr-101 to stimulate its activity PubMed
  • Protein family

  • protein kinase domain (according to Swiss-Prot)
  • Paralogous protein(s)

    Kinetic information

    Domains

  • contains three C-terminal PASTA domains (aa 356-424, 425-492, 493-559) (binds muropeptides) PubMed
  • Modification

  • phosphorylation on Thr-290 PubMed, autophosphorylation on multiple threonine residues PubMed
  • Cofactors

    Effectors of protein activity

  • activated by muropeptides PubMed
  • GpsB, DivIVA, and EzrA are required for stimulation of PrkC activity PubMed
  • Structure

  • 4EQM (intracellular domain of the Staphylococcus aureus enzyme, 51% identity) PubMed
  • 3PY9 (entire extra-cellular region of PrkC from Staphylococcus aureus) PubMed
  • 4X3F (intracellular domain of the Mycobacterium tuberculosis enzyme, 36% identity, 68% similarity) PubMed
  • Localization

  • inner spore membrane PubMed
  • membrane PubMed
  • division septum PubMed
  • Interactions

  • AbrB-PrkC PubMed
  • YvcK-PrkC PubMed
  • GpsB-PrkC PubMed
  • Additional information

    Expression and Regulation


    Operon

  • prpC-prkC-cpgA PubMed
  • Sigma factor

    Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • MGNA-B134 (yloP::erm), available at the NBRP B. subtilis, Japan
  • GP576 (spc), OMG302 (aphA3), available in Stülke lab
  • 1A820 ( prkC::erm), PubMed, available at BGSC
  • 1A963 (no resistance), PubMed, available at BGSC
  • 1A964 (no resistance), PubMed, available at BGSC
  • Expression vector

  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP832, available in Stülke lab
  • for expression/ purification of the kinase domain from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP849, available in Stülke lab
  • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1001, available in Stülke lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP825, available in Stülke lab
  • lacZ fusion

  • pGP829 (in pAC7), available in Stülke lab
  • GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Reviews

    Pompeo F, Foulquier E, Galinier A

    Impact of Serine/Threonine Protein Kinases on the Regulation of Sporulation in Bacillus subtilis

    Front Microbiol. 2016 Apr 20;7:568. doi: 10.3389/fmicb.2016.00568. eCollection 2016 Apr 20. Review. PubMed PMID: 27148245; PubMed Central PMCID: PMC4837961.
    Manuse S, Fleurie A, Zucchini L, Lesterlin C, Grangeasse C

    Role of eukaryotic-like serine/threonine kinases in bacterial cell division and morphogenesis

    FEMS Microbiol Rev. 2016 Jan;40(1):41-56. doi: 10.1093/femsre/fuv041. Epub 2015 Sep 30. Review. PubMed PMID: 26429880.
    Pereira SF, Goss L, Dworkin J

    Eukaryote-like serine/threonine kinases and phosphatases in bacteria

    Microbiol Mol Biol Rev. 2011 Mar;75(1):192-212. doi: 10.1128/MMBR.00042-10. Review. PubMed PMID: 21372323; PubMed Central PMCID: PMC3063355.
    Dworkin J, Shah IM

    Exit from dormancy in microbial organisms

    Nat Rev Microbiol. 2010 Dec;8(12):890-6. doi: 10.1038/nrmicro2453. Epub 2010 Oct 25. Review. PubMed PMID: 20972452.

    Phosphorylation of PrkC

    Gruszczyński P, Obuchowski M, Kaźmierkiewicz R

    Phosphorylation and ATP-binding induced conformational changes in the PrkC, Ser/Thr kinase from B

    subtilis. J Comput Aided Mol Des. 2010 Sep;24(9):733-47. doi: 10.1007/s10822-010-9370-4. Epub 2010 Jun 19. PubMed PMID: 20563625.
    Madec E, Stensballe A, Kjellström S, Cladière L, Obuchowski M, Jensen ON, Séror SJ

    Mass spectrometry and site-directed mutagenesis identify several autophosphorylated residues required for the activity of PrkC, a Ser/Thr kinase from Bacillus subtilis

    J Mol Biol. 2003 Jul 11;330(3):459-72. PubMed PMID: 12842463.

    Targets of PrkC-dependent phosphorylation

    Libby EA, Goss LA, Dworkin J

    The Eukaryotic-Like Ser/Thr Kinase PrkC Regulates the Essential WalRK Two-Component System in Bacillus subtilis

    PLoS Genet. 2015 Jun 23;11(6):e1005275. doi: 10.1371/journal.pgen.1005275. eCollection 2015 Jun 23. PubMed PMID: 26102633; PubMed Central PMCID: PMC4478028.
    Pompeo F, Foulquier E, Serrano B, Grangeasse C, Galinier A

    Phosphorylation of the cell division protein GpsB regulates PrkC kinase activity through a negative feedback loop in Bacillus subtilis

    Mol Microbiol. 2015 Jul;97(1):139-50. doi: 10.1111/mmi.13015. Epub 2015 Apr 24. PubMed PMID: 25845974.
    Shi L, Pigeonneau N, Ravikumar V, Dobrinic P, Macek B, Franjevic D, Noirot-Gros MF, Mijakovic I

    Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues

    Front Microbiol. 2014 Sep 17;5:495. doi: 10.3389/fmicb.2014.00495. eCollection 2014 Sep 17. PubMed PMID: 25278935; PubMed Central PMCID: PMC4166321.
    Foulquier E, Pompeo F, Freton C, Cordier B, Grangeasse C, Galinier A

    PrkC-mediated phosphorylation of overexpressed YvcK protein regulates PBP1 protein localization in Bacillus subtilis mreB mutant cells

    J Biol Chem. 2014 Aug 22;289(34):23662-9. doi: 10.1074/jbc.M114.562496. Epub 2014 Jul 10. PubMed PMID: 25012659; PubMed Central PMCID: PMC4156092.
    Kobir A, Poncet S, Bidnenko V, Delumeau O, Jers C, Zouhir S, Grenha R, Nessler S, Noirot P, Mijakovic I

    Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties

    Mol Microbiol. 2014 Jun;92(5):1129-41. doi: 10.1111/mmi.12617. Epub 2014 Apr 29. PubMed PMID: 24731262.
    Ravikumar V, Shi L, Krug K, Derouiche A, Jers C, Cousin C, Kobir A, Mijakovic I, Macek B

    Quantitative phosphoproteome analysis of Bacillus subtilis reveals novel substrates of the kinase PrkC and phosphatase PrpC

    Mol Cell Proteomics. 2014 Aug;13(8):1965-78. doi: 10.1074/mcp.M113.035949. Epub 2014 Jan 5. PubMed PMID: 24390483; PubMed Central PMCID: PMC4125730.
    Pietack N, Becher D, Schmidl SR, Saier MH, Hecker M, Commichau FM, Stülke J

    In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism

    J Mol Microbiol Biotechnol. 2010;18(3):129-40. doi: 10.1159/000308512. Epub 2010 Apr 13. PubMed PMID: 20389117.
    Shah IM, Dworkin J

    Induction and regulation of a secreted peptidoglycan hydrolase by a membrane Ser/Thr kinase that detects muropeptides

    Mol Microbiol. 2010 Mar;75(5):1232-43. doi: 10.1111/j.1365-2958.2010.07046.x. Epub 2010 Jan 12. PubMed PMID: 20070526.
    Absalon C, Obuchowski M, Madec E, Delattre D, Holland IB, Séror SJ

    CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis

    Microbiology. 2009 Mar;155(Pt 3):932-43. doi: 10.1099/mic.0.022475-0. PubMed PMID: 19246764.

    Phsiological role of PrkC

    Libby EA, Goss LA, Dworkin J

    The Eukaryotic-Like Ser/Thr Kinase PrkC Regulates the Essential WalRK Two-Component System in Bacillus subtilis

    PLoS Genet. 2015 Jun 23;11(6):e1005275. doi: 10.1371/journal.pgen.1005275. eCollection 2015 Jun 23. PubMed PMID: 26102633; PubMed Central PMCID: PMC4478028.
    Absalon C, Obuchowski M, Madec E, Delattre D, Holland IB, Séror SJ

    CpgA, EF-Tu and the stressosome protein YezB are substrates of the Ser/Thr kinase/phosphatase couple, PrkC/PrpC, in Bacillus subtilis

    Microbiology. 2009 Mar;155(Pt 3):932-43. doi: 10.1099/mic.0.022475-0. PubMed PMID: 19246764.
    Shah IM, Laaberki MH, Popham DL, Dworkin J

    A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments

    Cell. 2008 Oct 31;135(3):486-96. doi: 10.1016/j.cell.2008.08.039. PubMed PMID: 18984160; PubMed Central PMCID: PMC2892110.
    Madec E, Stensballe A, Kjellström S, Cladière L, Obuchowski M, Jensen ON, Séror SJ

    Mass spectrometry and site-directed mutagenesis identify several autophosphorylated residues required for the activity of PrkC, a Ser/Thr kinase from Bacillus subtilis

    J Mol Biol. 2003 Jul 11;330(3):459-72. PubMed PMID: 12842463.
    Madec E, Laszkiewicz A, Iwanicki A, Obuchowski M, Séror S

    Characterization of a membrane-linked Ser/Thr protein kinase in Bacillus subtilis, implicated in developmental processes

    Mol Microbiol. 2002 Oct;46(2):571-86. PubMed PMID: 12406230.
    Gaidenko TA, Kim TJ, Price CW

    The PrpC serine-threonine phosphatase and PrkC kinase have opposing physiological roles in stationary-phase Bacillus subtilis cells

    J Bacteriol. 2002 Nov;184(22):6109-14. PubMed PMID: 12399479; PubMed Central PMCID: PMC151969.

    Expression/ localization of PrkC

    Zheng L, Abhyankar W, Ouwerling N, Dekker HL, van Veen H, van der Wel NN, Roseboom W, de Koning LJ, Brul S, de Koster CG

    Bacillus subtilis Spore Inner Membrane Proteome

    J Proteome Res. 2016 Feb 5;15(2):585-94. doi: 10.1021/acs.jproteome.5b00976. Epub 2016 Jan 13. PubMed PMID: 26731423.
    Chen Y, Ray WK, Helm RF, Melville SB, Popham DL

    Levels of germination proteins in Bacillus subtilis dormant, superdormant, and germinating spores

    PLoS One. 2014 Apr 21;9(4):e95781. doi: 10.1371/journal.pone.0095781. eCollection 2014 Apr 21. PubMed PMID: 24752279; PubMed Central PMCID: PMC3994143.
    Iwanicki A, Hinc K, Seror S, Wegrzyn G, Obuchowski M

    Transcription in the prpC-yloQ region in Bacillus subtilis

    Arch Microbiol. 2005 Sep;183(6):421-30. Epub 2005 Oct 13. PubMed PMID: 16025310.

    Structure/ biochemistry of PrkC

    Berisio R, Squeglia F, Ruggiero A, Petraccone L, Stellato MI, Del Vecchio P

    Differential thermodynamic behaviours of the extra-cellular regions of two Ser/Thr PrkC kinases revealed by calorimetric studies

    Biochim Biophys Acta. 2015 May;1854(5):402-9. doi: 10.1016/j.bbapap.2015.02.001. Epub 2015 Feb 8. PubMed PMID: 25668224.
    Wagner T, Alexandre M, Duran R, Barilone N, Wehenkel A, Alzari PM, Bellinzoni M

    The crystal structure of the catalytic domain of the ser/thr kinase PknA from M

    tuberculosis shows an Src-like autoinhibited conformation. Proteins. 2015 May;83(5):982-8. doi: 10.1002/prot.24754. Epub 2015 Mar 25. PubMed PMID: 25586004.
    Arora G, Sajid A, Arulanandh MD, Misra R, Singhal A, Kumar S, Singh LK, Mattoo AR, Raj R, Maiti S, Basu-Modak S, Singh Y

    Zinc regulates the activity of kinase-phosphatase pair (BasPrkC/BasPrpC) in Bacillus anthracis

    Biometals. 2013 Oct;26(5):715-30. doi: 10.1007/s10534-013-9646-y. Epub 2013 Jun 22. PubMed PMID: 23793375.
    Rakette S, Donat S, Ohlsen K, Stehle T

    Structural analysis of Staphylococcus aureus serine/threonine kinase PknB

    PLoS One. 2012;7(6):e39136. doi: 10.1371/journal.pone.0039136. Epub 2012 Jun 11. PubMed PMID: 22701750; PubMed Central PMCID: PMC3372466.
    Squeglia F, Marchetti R, Ruggiero A, Lanzetta R, Marasco D, Dworkin J, Petoukhov M, Molinaro A, Berisio R, Silipo A

    Chemical basis of peptidoglycan discrimination by PrkC, a key kinase involved in bacterial resuscitation from dormancy

    J Am Chem Soc. 2011 Dec 28;133(51):20676-9. doi: 10.1021/ja208080r. Epub 2011 Dec 6. PubMed PMID: 22111897.
    Ruggiero A, Squeglia F, Marasco D, Marchetti R, Molinaro A, Berisio R

    X-ray structural studies of the entire extracellular region of the serine/threonine kinase PrkC from Staphylococcus aureus

    Biochem J. 2011 Apr 1;435(1):33-41. doi: 10.1042/BJ20101643. PubMed PMID: 21208192.
    '
    No login