SubtiBank SubtiBank
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Categories containing this gene/protein


phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons


ManR regulon

Gene


  • Coordinates on the chromosome (coding sequence): 1,272,725 -> 1,274,677
  • The protein


    Catalyzed reaction/ biological activity

  • transport and concomitant phosphorylation of mannose
  • Protein family

  • PTS permease, fructose/ mannitol permease (Fru) family PubMed
  • Paralogous protein(s)

  • FruA
  • Kinetic information

    Domains

    Modification

  • phosphorylation on Ser-365 PubMed
  • Cofactors

    Effectors of protein activity

    Structure

  • 2R48 (IIB domain)
  • Localization

  • membrane
  • Interactions

  • ManP-ManR, ManP-HPr
  • Additional information

    Expression and Regulation


    Operon

  • manP-manA-yjdF PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • induced by mannose (ManR) PubMed
  • repressed by glucose (ManR) PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed
  • Regulatory mechanism

  • ManR: transcription activation in the presence of mannose and absence of glucose PubMed
  • Additional information

    Biological materials


    Mutant

  • MGNA-A270 (yjdD::erm), available at the NBRP B. subtilis, Japan
  • Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Wenzel M, Altenbuchner J

    Development of a markerless gene deletion system for Bacillus subtilis based on the mannose phosphoenolpyruvate-dependent phosphotransferase system

    Microbiology. 2015 Oct;161(10):1942-9. doi: 10.1099/mic.0.000150. Epub 2015 Jul 31. PubMed PMID: 26238998.
    Wenzel M, Altenbuchner J

    The Bacillus subtilis mannose regulator, ManR, a DNA-binding protein regulated by HPr and its cognate PTS transporter ManP

    Mol Microbiol. 2013 May;88(3):562-76. doi: 10.1111/mmi.12209. Epub 2013 Apr 1. PubMed PMID: 23551403.
    de Jong IG, Veening JW, Kuipers OP

    Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation

    Environ Microbiol. 2012 Dec;14(12):3110-21. doi: 10.1111/j.1462-2920.2012.02892.x. Epub 2012 Oct 4. PubMed PMID: 23033921.
    Sun T, Altenbuchner J

    Characterization of a mannose utilization system in Bacillus subtilis

    J Bacteriol. 2010 Apr;192(8):2128-39. doi: 10.1128/JB.01673-09. Epub 2010 Feb 5. PubMed PMID: 20139185; PubMed Central PMCID: PMC2849456.
    Macek B, Mijakovic I, Olsen JV, Gnad F, Kumar C, Jensen PR, Mann M

    The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis

    Mol Cell Proteomics. 2007 Apr;6(4):697-707. Epub 2007 Jan 10. PubMed PMID: 17218307.
    Reizer J, Bachem S, Reizer A, Arnaud M, Saier MH Jr, Stülke J

    Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis

    Microbiology. 1999 Dec;145 ( Pt 12):3419-29. PubMed PMID: 10627040.
    Reizer J, Bachem S, Reizer A, Arnaud M, Saier MH Jr, Stülke J

    Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis

    Microbiology. 1999 Dec;145 ( Pt 12):3419-29. PubMed PMID: 10627040.
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