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Categories containing this gene/protein


utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

This gene is a member of the following regulons


The LicT regulon


Gene


  • Coordinates on the chromosome (coding sequence): 4,012,866 -> 4,013,699
  • Phenotypes of a mutant

  • no expression of the bglP-bglH operon
  • The protein


    Catalyzed reaction/ biological activity

  • binding to the mRNAs of bglS and the bglP-bglH operon, causes transcription antitermination (in presence of salicin and absence of glucose)
  • Protein family

  • BglG family of antiterminators (according to Swiss-Prot)
  • Paralogous protein(s)

  • SacY, GlcT, SacT
  • Kinetic information

    Domains

  • N-terminal RNA binding domain PubMed
  • 2 x PRD (PTS regulation domains) PubMed
  • Modification

  • phosphorylation at His-100 in PRD-1 by phosphorylated BglP, inhibits LicT antitermination activity
  • phosphorylation at His-207 and/or His-269 in PRD-2 by His-P-HPr, stimulates LicT antitermination activity
  • Cofactors

    Effectors of protein activity

    Structure

  • 1L1C (complex with RAT)
  • 1TLV (PRDs)
  • Localization

  • cytoplasm, even distribution in the absence of the inducer salicin, subpolar localization in the presence of salicin PubMed
  • Interactions

  • PtsH-LicT, BglP-LicT
  • Additional information

    Expression and Regulation


    Operon

  • licT-bglS PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

    Regulatory mechanism

    Additional information

    Biological materials


    Mutant

  • GP427 (licTS, erm), available in Jörg Stülke's lab
  • Expression vector

  • for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP165, available in Jörg Stülke's lab
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP315, available in Jörg Stülke's lab
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP572, available in Jörg Stülke's lab
  • lacZ fusion

    GFP fusion

  • GP1225 (spc, based on pGP1870), available in Jörg Stülke's lab
  • two-hybrid system

    FLAG-tag construct

  • GP1221 (spc, based on pGP1331), available in Jörg Stülke's lab
  • Antibody

    Labs working on this gene/protein


  • Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • Josef Deutscher, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
  • Michael Hecker, Greifswald, Germany Homepage
  • References


    Reviews

    Commichau FM, Stülke J

    Trigger enzymes: bifunctional proteins active in metabolism and in controlling gene expression

    Mol Microbiol. 2008 Feb;67(4):692-702. Epub 2007 Dec 11. Review. PubMed PMID: 18086213.
    Stülke J, Arnaud M, Rapoport G, Martin-Verstraete I

    PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria

    Mol Microbiol. 1998 Jun;28(5):865-74. Review. PubMed PMID: 9663674.

    Original publications

    Rothe FM, Wrede C, Lehnik-Habrink M, Görke B, Stülke J

    Dynamic localization of a transcription factor in Bacillus subtilis: the LicT antiterminator relocalizes in response to inducer availability

    J Bacteriol. 2013 May;195(10):2146-54. doi: 10.1128/JB.00117-13. Epub 2013 Mar 8. PubMed PMID: 23475962; PubMed Central PMCID: PMC3650534.
    Bahr T, Lüttmann D, März W, Rak B, Görke B

    Insight into bacterial phosphotransferase system-mediated signaling by interspecies transplantation of a transcriptional regulator

    J Bacteriol. 2011 Apr;193(8):2013-26. doi: 10.1128/JB.01459-10. Epub 2011 Feb 18. PubMed PMID: 21335451; PubMed Central PMCID: PMC3133047.
    Hübner S, Declerck N, Diethmaier C, Le Coq D, Aymerich S, Stülke J

    Prevention of cross-talk in conserved regulatory systems: identification of specificity determinants in RNA-binding anti-termination proteins of the BglG family

    Nucleic Acids Res. 2011 May;39(10):4360-72. doi: 10.1093/nar/gkr021. Epub 2011 Jan 28. PubMed PMID: 21278164; PubMed Central PMCID: PMC3105407.
    Schilling O, Herzberg C, Hertrich T, Vörsmann H, Jessen D, Hübner S, Titgemeyer F, Stülke J

    Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples

    Nucleic Acids Res. 2006;34(21):6102-15. Epub 2006 Oct 29. PubMed PMID: 17074746; PubMed Central PMCID: PMC1635312.
    Graille M, Zhou CZ, Receveur-Bréchot V, Collinet B, Declerck N, van Tilbeurgh H

    Activation of the LicT transcriptional antiterminator involves a domain swing/lock mechanism provoking massive structural changes

    J Biol Chem. 2005 Apr 15;280(15):14780-9. Epub 2005 Feb 7. PubMed PMID: 15699035.
    Ducat T, Declerck N, Kochoyan M, Déméné H

    1H, 15N and 13C backbone resonance assignments of the 40 kDa LicT-CAT-PRD1 protein

    J Biomol NMR. 2002 Aug;23(4):325-6. PubMed PMID: 12398354.
    Lindner C, Hecker M, Le Coq D, Deutscher J

    Bacillus subtilis mutant LicT antiterminators exhibiting enzyme I- and HPr-independent antitermination affect catabolite repression of the bglPH operon

    J Bacteriol. 2002 Sep;184(17):4819-28. PubMed PMID: 12169607; PubMed Central PMCID: PMC135283.
    Declerck N, Dutartre H, Receveur V, Dubois V, Royer C, Aymerich S, van Tilbeurgh H

    Dimer stabilization upon activation of the transcriptional antiterminator LicT

    J Mol Biol. 2001 Dec 7;314(4):671-81. PubMed PMID: 11733988.
    Tortosa P, Declerck N, Dutartre H, Lindner C, Deutscher J, Le Coq D

    Sites of positive and negative regulation in the Bacillus subtilis antiterminators LicT and SacY

    Mol Microbiol. 2001 Sep;41(6):1381-93. PubMed PMID: 11580842.
    van Tilbeurgh H, Le Coq D, Declerck N

    Crystal structure of an activated form of the PTS regulation domain from the LicT transcriptional antiterminator

    EMBO J. 2001 Jul 16;20(14):3789-99. PubMed PMID: 11447120; PubMed Central PMCID: PMC125546.
    van Tilbeurgh H, Le Coq D, Declerck N

    Crystal structure of an activated form of the PTS regulation domain from the LicT transcriptional antiterminator

    EMBO J. 2001 Jul 16;20(14):3789-99. PubMed PMID: 11447120; PubMed Central PMCID: PMC125546.
    Declerck N, Vincent F, Hoh F, Aymerich S, van Tilbeurgh H

    RNA recognition by transcriptional antiterminators of the BglG/SacY family: functional and structural comparison of the CAT domain from SacY and LicT

    J Mol Biol. 1999 Nov 26;294(2):389-402. PubMed PMID: 10610766.
    Lindner C, Galinier A, Hecker M, Deutscher J

    Regulation of the activity of the Bacillus subtilis antiterminator LicT by multiple PEP-dependent, enzyme I- and HPr-catalysed phosphorylation

    Mol Microbiol. 1999 Feb;31(3):995-1006. PubMed PMID: 10048041.
    Krüger S, Gertz S, Hecker M

    Transcriptional analysis of bglPH expression in Bacillus subtilis: evidence for two distinct pathways mediating carbon catabolite repression

    J Bacteriol. 1996 May;178(9):2637-44. PubMed PMID: 8626332; PubMed Central PMCID: PMC177989.
    Schnetz K, Stülke J, Gertz S, Krüger S, Krieg M, Hecker M, Rak B

    LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family

    J Bacteriol. 1996 Apr;178(7):1971-9. PubMed PMID: 8606172; PubMed Central PMCID: PMC177893.
    Krüger S, Hecker M

    Regulation of the putative bglPH operon for aryl-beta-glucoside utilization in Bacillus subtilis

    J Bacteriol. 1995 Oct;177(19):5590-7. PubMed PMID: 7559347; PubMed Central PMCID: PMC177369.
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