SubtiBank SubtiBank

Categories containing this gene/protein


phosphotransferase systems, utilization of specific carbon sources, phosphoproteins, sporulation proteins

This gene is a member of the following regulons


CcpA regulon, LicR regulon

Gene


  • Coordinates on the chromosome (coding sequence): 3,959,841 -> 3,960,173
  • The protein


    Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)
  • Protein family

  • PTS permease, lactose permease (Lac) family PubMed
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

  • 3K1S (IIA(Cel) from B. anthracis, 42% identity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • LicA-HPr, LicA-LicB
  • Additional information

    Expression and Regulation


    Operon

  • licB-licC-licA-licH PubMed
  • Sigma factor

  • SigA PubMed
  • Regulation

  • carbon catabolite repression (CcpA) PubMed
  • induction by oligomeric ß-glucosides (LicR)PubMed
  • expression during spore germination is increased under conditions of osmotic stress PubMed
  • Regulatory mechanism

  • CcpA: transcription repression PubMed
  • LicR: transcription activation PubMed
  • Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Nagler K, Krawczyk AO, De Jong A, Madela K, Hoffmann T, Laue M, Kuipers OP, Bremer E, Moeller R

    Identification of Differentially Expressed Genes during Bacillus subtilis Spore Outgrowth in High-Salinity Environments Using RNA Sequencing

    Front Microbiol. 2016 Oct 6;7:1564. eCollection 2016 Oct 6. PubMed PMID: 27766092; PubMed Central PMCID: PMC5052260.
    Inaoka T, Satomura T, Fujita Y, Ochi K

    Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis

    FEMS Microbiol Lett. 2009 Feb;291(2):151-6. doi: 10.1111/j.1574-6968.2008.01450.x. Epub 2008 Dec 11. PubMed PMID: 19087206.
    Tam le T, Eymann C, Albrecht D, Sietmann R, Schauer F, Hecker M, Antelmann H

    Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis

    Environ Microbiol. 2006 Aug;8(8):1408-27. PubMed PMID: 16872404.
    Reizer J, Bachem S, Reizer A, Arnaud M, Saier MH Jr, Stülke J

    Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis

    Microbiology. 1999 Dec;145 ( Pt 12):3419-29. PubMed PMID: 10627040.
    Tobisch S, Stülke J, Hecker M

    Regulation of the lic operon of Bacillus subtilis and characterization of potential phosphorylation sites of the LicR regulator protein by site-directed mutagenesis

    J Bacteriol. 1999 Aug;181(16):4995-5003. PubMed PMID: 10438772; PubMed Central PMCID: PMC93989.
    Tobisch S, Glaser P, Krüger S, Hecker M

    Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis

    J Bacteriol. 1997 Jan;179(2):496-506. PubMed PMID: 8990303; PubMed Central PMCID: PMC178721.
    Tobisch S, Glaser P, Krüger S, Hecker M

    Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis

    J Bacteriol. 1997 Jan;179(2):496-506. PubMed PMID: 8990303; PubMed Central PMCID: PMC178721.
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