SubtiBank SubtiBank

Categories containing this gene/protein


phosphotransferase systems, utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons


CcpA regulon, LevR regulon, SigL regulon

Gene


  • Coordinates on the chromosome (coding sequence): 2,762,395 -> 2,762,835
  • The protein


    Catalyzed reaction/ biological activity

  • Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine (according to Swiss-Prot)
  • Protein family

  • PTS permease, mannose permease (Man) family PubMed
  • Paralogous protein(s)

    Kinetic information

    Domains

    Modification

    Cofactors

    Effectors of protein activity

    Structure

    Localization

  • cytoplasm (according to Swiss-Prot)
  • Interactions

  • LevD-HPr, LevD-LevE
  • Additional information

    Expression and Regulation


    Operon

  • levD-levE-levF-levG-sacC PubMed
  • Sigma factor

  • SigL PubMed
  • Regulation

  • subject to carbon catabolite repression (CcpA, LevR) PubMed
  • induced in the presence of fructose (LevR) PubMed
  • Regulatory mechanism

  • CcpA: transcription repression PubMed
  • LevR: transcription activation PubMed
  • Additional information

    Biological materials


    Mutant

    Expression vector

    lacZ fusion

    GFP fusion

    two-hybrid system

    FLAG-tag construct

    Antibody

    Labs working on this gene/protein


    References


    Marciniak BC, Pabijaniak M, de Jong A, Dűhring R, Seidel G, Hillen W, Kuipers OP

    High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis

    BMC Genomics. 2012 Aug 17;13:401. doi: 10.1186/1471-2164-13-401. PubMed PMID: 22900538; PubMed Central PMCID: PMC3463425.
    Reizer J, Bachem S, Reizer A, Arnaud M, Saier MH Jr, Stülke J

    Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis

    Microbiology. 1999 Dec;145 ( Pt 12):3419-29. PubMed PMID: 10627040.
    Charrier V, Deutscher J, Galinier A, Martin-Verstraete I

    Protein phosphorylation chain of a Bacillus subtilis fructose-specific phosphotransferase system and its participation in regulation of the expression of the lev operon

    Biochemistry. 1997 Feb 4;36(5):1163-72. PubMed PMID: 9033408.
    Stülke J, Martin-Verstraete I, Charrier V, Klier A, Deutscher J, Rapoport G

    The HPr protein of the phosphotransferase system links induction and catabolite repression of the Bacillus subtilis levanase operon

    J Bacteriol. 1995 Dec;177(23):6928-36. PubMed PMID: 7592487; PubMed Central PMCID: PMC177562.
    Martin-Verstraete I, Stülke J, Klier A, Rapoport G

    Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon

    J Bacteriol. 1995 Dec;177(23):6919-27. PubMed PMID: 7592486; PubMed Central PMCID: PMC177561.
    Martin-Verstraete I, Stülke J, Klier A, Rapoport G

    Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon

    J Bacteriol. 1995 Dec;177(23):6919-27. PubMed PMID: 7592486; PubMed Central PMCID: PMC177561.
    Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G

    Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon

    J Mol Biol. 1990 Aug 5;214(3):657-71. PubMed PMID: 2117666.
    Débarbouillé M, Martin-Verstraete I, Kunst F, Rapoport G

    The Bacillus subtilis sigL gene encodes an equivalent of sigma 54 from gram-negative bacteria

    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9092-6. PubMed PMID: 1924373; PubMed Central PMCID: PMC52658.
    Débarbouillé M, Martin-Verstraete I, Klier A, Rapoport G

    The transcriptional regulator LevR of Bacillus subtilis has domains homologous to both sigma 54- and phosphotransferase system-dependent regulators

    Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2212-6. PubMed PMID: 1900939; PubMed Central PMCID: PMC51200.
    Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G

    Mutagenesis of the Bacillus subtilis "-12, -24" promoter of the levanase operon and evidence for the existence of an upstream activating sequence

    J Mol Biol. 1992 Jul 5;226(1):85-99. PubMed PMID: 1619665.
    No login